Dihexosylceramides Analysis Service

With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics has developed a reliable and reproducible method using highly sensitive LC-MS/MS method for the rapid identification and quantification of dihexosylceramide in different sample types, which can satisfy the needs of academic and industrial study in your lab.

Overview

Dihexosylceramides Targeted Lipidomics

Dihexosylceramide (CerG2 or DiHexCer) is a glycosphingolipid. Their structure consists of a ceramide backbone connected to a neutral disaccharide molecule. The ceramide backbone contains two hydrocarbon chains: long-chain bases connected to fatty acids through amide bonds. Fatty acids and long-chain bases are variable in length, are hydroxylated and contain double bonds.

Many dihexosylceramides are key intermediates in the biosynthesis of glycosphingolipids, which contain three or more sugar molecules, such as globulins and gangliosides. However, they are essential for cell function and are particularly important for the formation of lipid rafts in the cells of the innate immune system. They bind to pathogens and mediate immune and inflammatory responses. In addition, abnormal metabolism of dihexylceramide is associated with cancer, cardiovascular disease, inflammation and multiple sclerosis.

Currently, a reliable and reproducible method using highly sensitive LC-MS/MS platform for the identification and quantification of CerG2 in different sample types has been established by the scientists at Creative Proteomics, which can satisfy the needs of academic and industrial study in your lab.

Advantages of Our Dihexosylceramide Analysis Service

  • We routinely cover a rich variety of sample types, including cells, conditioned medium, subcellular compartments, exsome, tissue, blood, plasma, urine for our customer. Still, our services require only minimal sample amounts per analysis.
  • With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective CerG2 targeted lipidomics services.
  • Send us your sample and receive data in 2-4 weeks.

List of Detectable Dihexosylceramide at Creative Proteomics

DiHexCer 30:1;2DiHexCerDiHexCer 34:1;2

The Technical Process of Our Dihexosylceramide Analysis Service

The workflow of dihexosylceramide analysis serviceThe workflow of dihexosylceramide analysis service

Structural details: species level
Precision: pmol & mol%
Variants identified: >50
Delivery time: 2-4 weeks
Sample requirements
Blood/plasma: 500 ul/sample
Tissue: 200 mg/sample
Cells: 1x107/sample

  • A minimum of 6 replicate plates or wells per condition are required.
  • After the sample is collected, it will be stored at -80°C immediately and transported by dry ice.
  • Tubes should be clearly labeled with sample identifiers and include a hard copy of the list of samples to accompany the shipment. Please include all available sample information when shipping samples.

Deliverables

  • Experimental procedure
  • Parameters of liquid chromatography and MS
  • Purity analysis report
  • MS raw data files and MS data quality checks
  • Hexylceramide quantitative result data
  • Custom analysis report

Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, we provide reliable, fast and cost-effective CerG2 analysis services. If you have any questions about our CerG2 targeted lipidomics services, please contact us.

* Our services can only be used for research purposes and Not for clinical use.

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