With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics has developed a reliable and reproducible method using highly sensitive LC-MS/MS method for the rapid identification and quantification of dihexosylceramide in different sample types, which can satisfy the needs of academic and industrial study in your lab.
Dihexosylceramide (CerG2 or DiHexCer) is a glycosphingolipid. Their structure consists of a ceramide backbone connected to a neutral disaccharide molecule. The ceramide backbone contains two hydrocarbon chains: long-chain bases connected to fatty acids through amide bonds. Fatty acids and long-chain bases are variable in length, are hydroxylated and contain double bonds.
Many dihexosylceramides are key intermediates in the biosynthesis of glycosphingolipids, which contain three or more sugar molecules, such as globulins and gangliosides. However, they are essential for cell function and are particularly important for the formation of lipid rafts in the cells of the innate immune system. They bind to pathogens and mediate immune and inflammatory responses. In addition, abnormal metabolism of dihexylceramide is associated with cancer, cardiovascular disease, inflammation and multiple sclerosis.
Currently, a reliable and reproducible method using highly sensitive LC-MS/MS platform for the identification and quantification of CerG2 in different sample types has been established by the scientists at Creative Proteomics, which can satisfy the needs of academic and industrial study in your lab.
The workflow of dihexosylceramide analysis service
Structural details: species level
Precision: pmol & mol%
Variants identified: >50
Delivery time: 2-4 weeks
Blood/plasma: 500 ul/sample
Tissue: 200 mg/sample
Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, we provide reliable, fast and cost-effective CerG2 analysis services. If you have any questions about our CerG2 targeted lipidomics services, please contact us.