Lipidomics Platform & Instrumentation

Empowering High-Precision Lipid Analysis with Advanced Mass Spectrometry and Rigorous QC

At Creative Proteomics, our lipidomics platform delivers accurate, publication-ready data to help you confidently profile lipid species, validate biomarkers, or explore structural diversity. Powered by high-end mass spectrometry and rigorous quality control, we help researchers overcome challenges in sensitivity, reproducibility, and data interpretation.

Key Advantages:

  • Broad coverage of >1,000 lipid species
  • Targeted, untargeted, and structural workflows
  • Orbitrap, QTOF, TIMS, and triple quadrupole systems
  • Stable isotope-labeled internal standards
  • Bioinformatics-ready outputs: PCA, volcano plots, enrichment maps
  • Transparent QA/QC with internal controls and RSD thresholds
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  • Technologies & Analytical Methods
  • Core Instrumentation
  • How to Start
  • Results You Receive
  • Data Quality

Technologies & Analytical Methods

Robust lipid profiling powered by optimized chromatographic separations, multi-mode ionization, and accurate mass detection.

Lipidomics Analysis Strategies

Targeted Lipidomics

For absolute quantification of predefined lipid species using triple quadrupole LC-MS/MS.

  • Supports biomarker validation, clinical translation, and standardized panels.

Untargeted Lipidomics

For global profiling of lipidomes with high-resolution mass spectrometry (e.g., Orbitrap, QTOF).

  • Ideal for biomarker discovery, pathway exploration, and comparative studies.

Structural Lipidomics

For in-depth analysis of lipid isomers and subclass differentiation via MS/MS and ion mobility.

  • Resolves structural variants (e.g., sn-position, double bond location).

Chromatographic Separation Modes

RP-UHPLCHILICNormal Phase LC
For non-polar lipids: TAG, DAG, CE, FAFor polar lipids: PC, PE, PI, PS, SMFor class-based profiling or niche assays
C18-based, high-resolution LCAmide/HILIC columns for class separationLegacy or custom lipidomics workflows
Common in both targeted and untargetedEnhances subclass coverageLimited use, for special lipidomics

Each method is matched to sample type and project goal to maximize class coverage and detection resolution.

Ionization & Mass Detection Modes

Quantification Approaches

MethodDescriptionUse CasePerformance
Relative QuantificationPeak area normalization across samplesComparative studies, discoveryCV < 15%
Absolute QuantificationStable isotope-labeled internal standards (e.g., SPLASH Lipidomix)Biomarker validation, PK studiesR² > 0.99, wide dynamic range

We employ standard curves and internal calibrants to ensure consistent quantitation across batches.

Core Instrumentation

Thermo Scientific Orbitrap Exploris 480

Thermo Scientific Orbitrap Exploris 480

Parameter Highlights

  • Resolving power: up to 480,000 (FWHM at m/z 200)
  • Mass accuracy: <1 ppm (internal calibration)
  • Scan modes: Full MS, MS² (HCD), DDA, DIA
  • Polarity switching: yes (fast)

Analytical Performance

  • LOD: ~0.5–1 ng/mL (lipid class dependent)
  • Dynamic range: 5–6 orders of magnitude
  • Isomer resolution: MS/MS fragmentation + accurate mass

Lipid Classes Supported

  • Glycerophospholipids, Sphingolipids, Sterols
  • Oxidized lipids, Ether lipids, rare species

Use Cases

  • Untargeted lipid profiling
  • Biomarker discovery
  • Pathway enrichment + isomer annotation
Agilent 6495C Triple Quadrupole LC-MS

Agilent 6495C Triple Quadrupole LC-MS

Parameter Highlights

  • Scan modes: MRM, dynamic MRM
  • Polarity switching: yes
  • LOD: as low as 10 fg on-column
  • Response time: <5 ms

Analytical Performance

  • Dynamic range: 6–7 orders of magnitude
  • Quantification: absolute, isotope-labeled standard support
  • CV: <10% inter/intra-batch

Lipid Classes Supported

  • Eicosanoids, oxylipins, SPMs, short-chain lipids
  • Lysophospholipids, DAG, Ceramides

Use Cases

  • Targeted lipid quantification (e.g. inflammation mediators)
  • Clinical lipid panels
  • High-sensitivity biomarker validation
Bruker Daltonics timsTOF Pro

Bruker Daltonics timsTOF Pro

Parameter Highlights

  • TIMS + TOF integration
  • PASEF acquisition: >100 Hz
  • CCS value generation for structural lipids
  • MS² coverage: full-range

Analytical Performance

  • Isomer discrimination by ion mobility
  • LOD: ~0.5–2 ng/mL
  • Quantitative reproducibility: CV <15%

Lipid Classes Supported

  • Isomeric GPs, ether lipids, oxidized PUFAs
  • Chain-length and position-differentiated species

Use Cases

  • Isomer-level lipidomics
  • Oxidized lipid structural analysis
  • Lipid network annotation
SCIEX 6500+ Triple Quad LC-MS/MS

SCIEX 6500+ Triple Quad LC-MS/MS

Parameter Highlights

  • IonDrive™ turbo V source
  • MRM acquisition at >600 transitions/second
  • High-pressure chromatography compatible

Analytical Performance

  • LOD: sub-femtomole
  • Dynamic range: >10⁶
  • Quant CV: <12% with internal standards

Lipid Classes Supported

  • Oxylipins, ceramides, acylcarnitines, PEs, PIs

Use Cases

  • Targeted quant panels
  • Toxic lipid biomarker studies
  • Fast and reliable absolute quantification
Agilent 1290 Infinity II UHPLC System

Agilent 1260/1290 Infinity II UHPLC

Parameter Highlights

  • Up to 1300 bar backpressure
  • Compatible with RP, HILIC, NP columns
  • Auto-sampler: high-throughput loading

Analytical Performance

  • Class-based separation performance
  • Reproducible retention (CV < 0.5%)
  • Minimal carryover with optimized wash cycles

Lipid Classes Supported

  • Neutral lipids, phospholipids, lysolipids
  • Compatible with both targeted and untargeted workflows

Use Cases

  • Front-end LC separation
  • Large-scale lipidomic screening
  • Co-elution resolution in complex matrices
Thermo TSQ Altis Triple Quadrupole

Thermo TSQ Altis Triple Quadrupole

Parameter Highlights

  • MRM/PRM with ultra-fast polarity switching
  • Active exhaust & ion transfer optics
  • High cycle rate for multiplex quantitation

Analytical Performance

  • LOD: sub-ng/mL for most lipid panels
  • R² > 0.995 across 5-log range
  • Stable isotope compatibility

Lipid Classes Supported

  • Fatty acids, TAGs, SM, LPC, PGs

Use Cases

  • Lipid panel quantification
  • Nutritional lipidomics
  • Method validation for regulatory studies
Bruker ultrafleXtreme MALDI-TOF/TOF Imaging

Bruker ultrafleXtreme MALDI-TOF/TOF Imaging

Parameter Highlights

  • MALDI-MS imaging resolution: up to 20 µm
  • Dual TOF configuration
  • MS/MS imaging support

Analytical Performance

  • Tissue-specific lipid mapping
  • In situ lipid distribution
  • Fast sample prep (no derivatization)

Lipid Classes Supported

  • Phospholipids, sphingolipids, sulfatides, gangliosides

Use Cases

  • Spatial lipidomics in tissues
  • Tumor lipid microdomain profiling

How to Start Your Lipidomics Project

01

Contact Us

Tell us your goals & sample

02

Get a Quote

We provide a tallored quotation

03

Ship Samples

Send your samples with proper handling

04

Analysis

We provide lipid profiling & QC

05

Get Results

Receive annotated data & bioinformatics

Explore how our advanced lipid analysis platform can support your research goals

Download Our Lipidomics Solution Brochure

What Results Will You Receive?

We understand that downstream usability is just as important as data generation. Our lipidomics platform provides clean, publication-ready datasets tailored for biomarker discovery, mechanistic studies, or regulatory submission.

Typical Data Deliverables Include:

  • Lipid concentration tables (absolute/relative, ng/mL or mol%)
  • Lipid species annotation with class, acyl chain, m/z
  • MS/MS spectral fragment mapping (CID/HCD)
  • Multivariate statistics: PCA, volcano, PLS-DA
  • Lipid class distribution plots and enrichment pathways
  • QA/QC performance metrics for transparency
  • Exportable formats: Excel, PDF, mzML, annotated figures

How Do We Ensure Data Quality?

High-confidence lipidomics begins with a rigorous quality control framework. Every project is processed with internal standards, spike-in controls, and method validation to ensure repeatability and accuracy.

Our QC Strategy Covers:

Control ElementDescription
Internal StandardsClass-specific isotope-labeled mix (e.g., SPLASH) added to every sample
Pooled QC SamplesInserted every 10 runs to monitor precision and drift
Reproducibility ThresholdsRSD <15% across biological and technical replicates
System SuitabilityVerified daily by standard response and retention metrics
Linearity & Calibration4–6 point standard curve validation, R² > 0.99

Resource:

* Our services can only be used for research purposes and Not for clinical use.

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