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Lipidomics Platform & Instrumentation

Empowering High-Precision Lipid Analysis with Advanced Mass Spectrometry and Rigorous QC

At Creative Proteomics, our lipidomics platform delivers accurate, publication-ready data to help you confidently profile lipid species, validate biomarkers, or explore structural diversity. Powered by high-end mass spectrometry and rigorous quality control, we help researchers overcome challenges in sensitivity, reproducibility, and data interpretation.

Key Advantages:

Broad coverage of >1,000 lipid species
Targeted, untargeted, and structural workflows
Orbitrap, QTOF, TIMS, and triple quadrupole systems
Stable isotope-labeled internal standards
Bioinformatics-ready outputs: PCA, volcano plots, enrichment maps
Transparent QA/QC with internal controls and RSD thresholds

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Technologies & Analytical Methods
Core Instrumentation
How to Start
Results You Receive
Data Quality

Technologies & Analytical Methods

Robust lipid profiling powered by optimized chromatographic separations, multi-mode ionization, and accurate mass detection.

Lipidomics Analysis Strategies

Targeted Lipidomics

For absolute quantification of predefined lipid species using triple quadrupole LC-MS/MS.

Supports biomarker validation, clinical translation, and standardized panels.

Untargeted Lipidomics

For global profiling of lipidomes with high-resolution mass spectrometry (e.g., Orbitrap, QTOF).

Ideal for biomarker discovery, pathway exploration, and comparative studies.

Structural Lipidomics

For in-depth analysis of lipid isomers and subclass differentiation via MS/MS and ion mobility.

Resolves structural variants (e.g., sn-position, double bond location).

Chromatographic Separation Modes

RP-UHPLC	HILIC	Normal Phase LC
For non-polar lipids: TAG, DAG, CE, FA	For polar lipids: PC, PE, PI, PS, SM	For class-based profiling or niche assays
C18-based, high-resolution LC	Amide/HILIC columns for class separation	Legacy or custom lipidomics workflows
Common in both targeted and untargeted	Enhances subclass coverage	Limited use, for special lipidomics

Each method is matched to sample type and project goal to maximize class coverage and detection resolution.

Ionization & Mass Detection Modes

Electrospray Ionization (ESI) – Soft ionization for intact lipid detection
Polarity Switching – Enables detection of both positive and negative ion mode lipids in a single run
Trapped Ion Mobility Spectrometry (TIMS) – Separates isobaric and isomeric lipid species based on gas-phase mobility
MS/MS Fragmentation (CID/HCD) – Provides acyl chain and headgroup structural information

Quantification Approaches

Method	Description	Use Case	Performance
Relative Quantification	Peak area normalization across samples	Comparative studies, discovery	CV < 15%
Absolute Quantification	Stable isotope-labeled internal standards (e.g., SPLASH Lipidomix)	Biomarker validation, PK studies	R² > 0.99, wide dynamic range

We employ standard curves and internal calibrants to ensure consistent quantitation across batches.

Core Instrumentation

Thermo Scientific Orbitrap Exploris 480

Parameter Highlights

Resolving power: up to 480,000 (FWHM at m/z 200)
Mass accuracy: <1 ppm (internal calibration)
Scan modes: Full MS, MS² (HCD), DDA, DIA
Polarity switching: yes (fast)

Analytical Performance

LOD: ~0.5–1 ng/mL (lipid class dependent)
Dynamic range: 5–6 orders of magnitude
Isomer resolution: MS/MS fragmentation + accurate mass

Lipid Classes Supported

Glycerophospholipids, Sphingolipids, Sterols
Oxidized lipids, Ether lipids, rare species

Use Cases

Untargeted lipid profiling
Biomarker discovery
Pathway enrichment + isomer annotation

Agilent 6495C Triple Quadrupole LC-MS

Parameter Highlights

Scan modes: MRM, dynamic MRM
Polarity switching: yes
LOD: as low as 10 fg on-column
Response time: <5 ms

Analytical Performance

Dynamic range: 6–7 orders of magnitude
Quantification: absolute, isotope-labeled standard support
CV: <10% inter/intra-batch

Lipid Classes Supported

Eicosanoids, oxylipins, SPMs, short-chain lipids
Lysophospholipids, DAG, Ceramides

Use Cases

Targeted lipid quantification (e.g. inflammation mediators)
Clinical lipid panels
High-sensitivity biomarker validation

Bruker Daltonics timsTOF Pro

Parameter Highlights

TIMS + TOF integration
PASEF acquisition: >100 Hz
CCS value generation for structural lipids
MS² coverage: full-range

Analytical Performance

Isomer discrimination by ion mobility
LOD: ~0.5–2 ng/mL
Quantitative reproducibility: CV <15%

Lipid Classes Supported

Isomeric GPs, ether lipids, oxidized PUFAs
Chain-length and position-differentiated species

Use Cases

Isomer-level lipidomics
Oxidized lipid structural analysis
Lipid network annotation

SCIEX 6500+ Triple Quad LC-MS/MS

Parameter Highlights

IonDrive™ turbo V source
MRM acquisition at >600 transitions/second
High-pressure chromatography compatible

Analytical Performance

LOD: sub-femtomole
Dynamic range: >10⁶
Quant CV: <12% with internal standards

Lipid Classes Supported

Oxylipins, ceramides, acylcarnitines, PEs, PIs

Use Cases

Targeted quant panels
Toxic lipid biomarker studies
Fast and reliable absolute quantification

Agilent 1260/1290 Infinity II UHPLC

Parameter Highlights

Up to 1300 bar backpressure
Compatible with RP, HILIC, NP columns
Auto-sampler: high-throughput loading

Analytical Performance

Class-based separation performance
Reproducible retention (CV < 0.5%)
Minimal carryover with optimized wash cycles

Lipid Classes Supported

Neutral lipids, phospholipids, lysolipids
Compatible with both targeted and untargeted workflows

Use Cases

Front-end LC separation
Large-scale lipidomic screening
Co-elution resolution in complex matrices

Thermo TSQ Altis Triple Quadrupole

Parameter Highlights

MRM/PRM with ultra-fast polarity switching
Active exhaust & ion transfer optics
High cycle rate for multiplex quantitation

Analytical Performance

LOD: sub-ng/mL for most lipid panels
R² > 0.995 across 5-log range
Stable isotope compatibility

Lipid Classes Supported

Fatty acids, TAGs, SM, LPC, PGs

Use Cases

Lipid panel quantification
Nutritional lipidomics
Method validation for regulatory studies

Bruker ultrafleXtreme MALDI-TOF/TOF Imaging

Parameter Highlights

MALDI-MS imaging resolution: up to 20 µm
Dual TOF configuration
MS/MS imaging support

Analytical Performance

Tissue-specific lipid mapping
In situ lipid distribution
Fast sample prep (no derivatization)

Lipid Classes Supported

Phospholipids, sphingolipids, sulfatides, gangliosides

Use Cases

Spatial lipidomics in tissues
Tumor lipid microdomain profiling

How to Start Your Lipidomics Project

Contact Us

Tell us your goals & sample

Get a Quote

We provide a tallored quotation

Ship Samples

Send your samples with proper handling

Analysis

We provide lipid profiling & QC

Get Results

Receive annotated data & bioinformatics

Explore how our advanced lipid analysis platform can support your research goals

Download Our Lipidomics Solution Brochure

What Results Will You Receive?

We understand that downstream usability is just as important as data generation. Our lipidomics platform provides clean, publication-ready datasets tailored for biomarker discovery, mechanistic studies, or regulatory submission.

Typical Data Deliverables Include:

Lipid concentration tables (absolute/relative, ng/mL or mol%)
Lipid species annotation with class, acyl chain, m/z
MS/MS spectral fragment mapping (CID/HCD)
Multivariate statistics: PCA, volcano, PLS-DA
Lipid class distribution plots and enrichment pathways
QA/QC performance metrics for transparency
Exportable formats: Excel, PDF, mzML, annotated figures

How Do We Ensure Data Quality?

High-confidence lipidomics begins with a rigorous quality control framework. Every project is processed with internal standards, spike-in controls, and method validation to ensure repeatability and accuracy.

Our QC Strategy Covers:

Control Element	Description
Internal Standards	Class-specific isotope-labeled mix (e.g., SPLASH) added to every sample
Pooled QC Samples	Inserted every 10 runs to monitor precision and drift
Reproducibility Thresholds	RSD <15% across biological and technical replicates
System Suitability	Verified daily by standard response and retention metrics
Linearity & Calibration	4–6 point standard curve validation, R² > 0.99

Resource:

* Our services can only be used for research purposes and Not for clinical use.

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