Cytidine Diphosphate Diacylglycerol Analysis Service
With extensive experience and state-of-the-art technologies, Creative Proteomics use highly sensitive LC-MS/MS method for the rapid identification and quantification of cytidine diphosphate diacylglycerol in different sample types, which can satisfy the needs of academic and industrial study.
Overview
Cytidine diphosphate diacylglycerol (CDP-DAG) is a key intermediate in the synthesis of phosphatidylinositol (PI) and cardiolipin (CL). Both PI and CL have highly specialized roles in cells. PI can be phosphorylated and these phosphorylated derivatives play major roles in signal transduction, membrane traffic, and maintenance of the actin cytoskeletal network. CL is the signature lipid of mitochondria and has a plethora of functions including maintenance of cristae morphology, mitochondrial fission, and fusion and for electron transport chain super complex formation. Further, CDP-DAG is linked to lipid droplet size in adipocytes. In human tumor tissues, increased CDP-DAG enhanced tumor proliferation.
The combination of MS instrument and liquid chromatography (LC) can effectively separate target lipid molecules from complex samples. High-quality and accurate lipid level analysis helps to study the changes in the content of CDP-DAG under physiological and other pathological conditions, and accelerate the analysis of biomarkers and related disease mechanisms.
Advantages of Our CDP-DAG Analysis Service
- One-stop services, including lipid extraction, lipid detection and data analysis. Cutting-edge apparatuses, reliable data and reproducible process.
- Creative Proteomics provides a detailed report to our clients, including experiment procedures and parameters of instruments, MS data with putative identification based on the m/z ratio of the analytes, differential analysis of lipids between treatment groups if needed.
- Our project is a very complex study that requires a high degree of sensitivity and accuracy. Creative Proteomics has molecular biologists, mass spectrometry specialists, bioinformatics experts, and lipidomics data scientists. We are well-prepared and positioned to foster collaboration in the fast-developing field of lipidomics.
List of Detectable CDP-DAG at Creative Proteomics
| CDP-DAG | CDP-DAG 34:1;0 | CDP-DAG 36:2;0 |
The Technical Process of Our CDP-DAG Analysis Service
Fig 2. The workflow of cytidine diphosphate diacylglycerol analysis service
Structural details: species level
Precision: pmol & mol%
Variants identified: <10
Delivery time: 2-4 weeks
Sample requirements
Blood/plasma: 500 ul/sample
Tissue: 200 mg/sample
Cells: 1x107/sample
- A minimum of 3 replicate plates or wells per condition are required.
- After the sample is collected, it will be stored at -80°C immediately and transported by dry ice.
- Tubes should be clearly labeled with sample identifiers and include a hard copy of the list of samples to accompany the shipment. Please include all available sample information when shipping samples.
Deliverables
- Experimental procedure
- Parameters of liquid chromatography and MS
- Purity analysis report
- MS raw data files and MS data quality checks
- Hexylceramide quantitative result data
- Custom analysis report
With years of experience in lipidomics analysis services, Creative Proteomics has a good reputation in supporting the detection and quantification of a variety of lipids. Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, we provide reliable, fast and cost-effective CDP-DAG analysis services. If you have any questions about our CDP-DAG targeted lipidomics services, please contact us.
* Our services can only be used for research purposes and Not for clinical use.
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