The lipid fraction only accounts for 5% of the total components of human milk, but the lipids in human milk are very important for babies. They are not only the main source of energy for the growth of babies, but also play a role in immune interaction and service. In addition, it is also related to infant nerve and retinal development.
The lipid group of human milk is complex. Lipids are strategically packaged as milk fat globules (MFG). MFG wraps triglycerides (TAGs) in the core, accounting for 98-99% of the total human milk lipid group. The surrounding MFG membrane is composed of other more polar lipids, such as cholesterol and phospholipids.
The content of total lipids in human milk varies greatly, during the day, between breasts, between women, and throughout the lactation period.
With the variability of human milk lipids, the complexity and lipid hydrophobicity of the milk matrix increase the difficulty of comprehensive lipidomics analysis.
Creative Proteomics provides reliable, rapid and cost-effective human milk untargeted lipidomics with GC/LC-QTOF-MS, which has the capacity to differentiate isomeric TAGs based on their structural arrangements and potentially identify more individual TAGs at once, as has been achieved for other lipid species. We also offer other mammals milk untargeted lipidomics services, including cow, macaque, goats, yaks, and pigs.
Creative Proteomics is based on the liquid-mass spectrometry (LC-MS) unbiased detection of all lipid molecules in milk samples as much as possible. We use statistical analysis to screen differential lipid molecules, discover the relative relationship between lipid metabolism changes and physiological and pathological changes, and reveal the mechanism of lipids in various life activities.
Fig 1. The workflow of milk untargeted lipidomics. (Villaseñor, A et al., 2014)
Due to these variations of both the total lipid content and lipid composition, lipidomic analysis at any given time has the potential to be very different. We suggest defining the research question and then determining the appropriate samples in order to define and standardize sampling to minimize variables and confounding factors.
Maintaining the integrity of a milk sample is carried out by freezing samples at temperatures such as −20 °C, −70 °C or −80 °C.
If you have any questions about our milk lipidomics service, please contact us.