With extensive experience and state-of-the-art technologies, Creative Proteomics use highly sensitive LC-MS/MS method for the rapid identification and quantification of globoside in different sample types, which can satisfy the needs of academic and industrial study.
A globoside is a type of glycosphingolipid with more than one sugar as the side chain (or R group) of ceramide. The sugars are usually a combination of N-acetylgalactosamine, D-glucose or D-galactose. Recent research shows that globoside has many biological functions. Gb3 globosides specifically interact with the cellular "death receptor" FasR. When paired to a Gb3, FasR transduces a cell death signal derived from activation of the caspase-8 cascade. A genetic defect in the enzyme alpha-galactosidase leads to accumulation of Gb3, causing a lysosomal storage disease. Further, Gb3 are a component of the cell membrane where they bind to Shigatoxins facilitating their entry into cells. Gb4 glycoside is the main glycolipid found in the cell membrane of human red blood cells. There, they are essential for cell interactions including blood type determinants, which determine the adaptability of donor blood to patients. Each of the three different blood group antigens is defined by the variation of the oligosaccharide unit. Gb4 lipid also acts as a receptor for parvovirus B19, which infects rapidly dividing intestinal cells and bone marrow, causing children.
The combination of MS instrument and liquid chromatography (LC) can effectively separate target lipid molecules from complex samples. High-quality and accurate lipid level analysis helps to study the changes in the content of globoside under physiological and other pathological conditions, and accelerate the analysis of biomarkers and related disease mechanisms.
|Gb3||Gb3 32:1;2||Gb3 36:1;2|
|Gb4||Gb4 36:1;2||Gb4 44:1;2|
Fig 2. The workflow of globoside analysis service
Structural details: species level
Precision: pmol & mol%
Variants identified: >10
Delivery time: 2-4 weeks
Blood/plasma: 500 ul/sample
With years of experience in lipidomics analysis services, Creative Proteomics has a good reputation in supporting the detection and quantification of a variety of lipids. Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, we provide reliable, fast and cost-effective globoside analysis services. If you have any questions about our globoside targeted lipidomics services, please contact us.