Targeted Lipidomics Service

  • Service Details
  • Case Study

Why Choose Targeted Lipidomics?

Lipids are biomolecules that are soluble in non-polar solvents, including fats, sterols, fat-soluble vitamins (such as vitamins A, D, E, and K), monoglycerides, diglycerides, triglycerides, and sheaths Lipids and phospholipids and so on. It is the main component that constitutes the biomembrane, and regulates the signal transduction process of cell growth, differentiation, senescence, and programmed death. It is also involved in physiological functions such as body growth, health, intellectual development, and memory. In addition, it can also affect the occurrence and development of various diseases such as tumor, cardiovascular, endocrine, immune, and nervous system.

When you want to study a specific target lipid or lipid class, you can choose targeted lipidomics. Precise method development allows for the inclusion of equal amounts of isotope-labeled or odd-chain internal standards selected from customizable lipid panels. Lipids from each sample are extracted using standardized protocols and analyzed by GC/LC-MS/MS. Analyte concentrations are determined from calibration curves prepared from authentic standards. This approach is especially useful for quantifying lipids present at low levels in samples, such as endogenous bioactive lipids (e.g., prostaglandins, leukotrienes, or endocannabinoids). Standard statistical analysis tools can reveal changing trends in lipid species among experimental groups, which can be visualized with a heat map or other available graphical tools.

Creative Proteomics provides reliable, fast, and cost-effective targeted lipidomics to accelerate your project.

Our Targeted Lipidomics Services

Liquid chromatography or gas chromatography hyphenated to triple quadrupole mass spectrometers for targeted lipidomics.

  • Shimadzu Nexera X2 U-HPLC System and AB Sciex 6500 QTRAP.
  • Thermo Scientific TSQ 9000 triple quadrupole GC-MS/MS system.

The protocol workflow of targeted lipidomicsFig1. The protocol workflow of targeted lipidomics (Creative Proteomics)

Featured Lipid Analysis Service

Sample Requirements for Targeted Lipidomics

Sample TypeSample CollectionLysis MethodRecommended QuantityAdditional Considerations
TissuesHomogenization for cell releaseTissue-specific lysis protocols10-50 mg tissueTissue-specific considerations; avoid contamination during handling.
Cell CultureHarvesting with trypsin or other methodsSpecialized buffers for efficient lysisVaries based on culture dishConsistent culture conditions and avoiding contamination are crucial.
Plasma/SerumBlood collection with anticoagulantsProtein precipitation or organic extraction100-500 μLImmediate sample processing to prevent lipid alterations.
Cell PelletsCentrifugation and washingSpecialized detergents for efficient lysis1-5 x 10^6 cellsOptimize washing steps to minimize cell residue.
LipoproteinsUltracentrifugation or density gradientOrganic extraction or specialized methods50-200 μLHandle lipoproteins with care to prevent structural alterations.
ExosomesUltracentrifugation or precipitationSpecialized extraction methods100-500 μLMaintain proper exosome isolation techniques for accurate analysis.
Breast MilkCollection in clean containersLipid extraction from milk5-10 mLMinimize contamination during collection and processing.
Skin BiopsyBiopsy procedureTissue-specific lysis protocols5-10 mg tissuePreserve sample integrity and prevent degradation.
UrineMidstream collection in sterile containersOrganic extraction or precipitation5-10 mLProcess samples promptly to prevent lipid changes.
FecesFresh collection in airtight containersHomogenization and organic extraction100-500 mgMinimize oxygen exposure to maintain sample stability.
PlantsHarvest and flash freezeTissue-specific lysis protocols50-200 mgRapid freezing preserves lipid profiles in plant tissues.
MicroorganismsCulture or direct collectionCell lysis and extraction methodsVaries based on biomassChoose appropriate lysis methods for different microorganisms.
FoodHomogenization or extractionSolvent-based extraction methodsVaries based on food typeTailor extraction methods to the characteristics of the food sample.

Why Choose Us?

  • Precision and Specificity: Achieve precise quantification of a targeted set of lipids with accuracy within a range of 0.1 to 1 nanomoles per gram of tissue, ensuring specificity in identifying and quantifying lipid species.
  • Quantitative Accuracy: Attain accurate quantification with a coefficient of variation (CV) below 5% for targeted lipid concentrations, demonstrating the service's ability to reliably measure dynamic changes in lipid profiles.
  • High Sensitivity: Detect low-abundance lipid species, reaching a detection limit as low as 0.01 nanomoles per gram of sample, showcasing exceptional sensitivity in identifying less abundant lipid molecules.
  • Comparative Studies: Conduct comparative studies with a precision in relative quantification of ±2% between different sample groups, allowing for robust comparisons of specific lipid levels across conditions.
  • Validation of Biomarkers: Validate lipid biomarkers with a high correlation coefficient (r) exceeding 0.95 between the targeted lipidomics results and established biomarker levels, confirming the reliability of identified lipid markers.
  • Robust and Reproducible Results: Ensure robust and reproducible results with inter-assay variability below 3%, demonstrating the service's consistency in delivering reliable data across multiple experiments.
  • Comprehensive Reporting: Receive comprehensive reports that include detailed information on identified lipid species, their concentrations, and statistical analyses. The report format provides clarity, aiding researchers in interpreting and utilizing the data effectively.

If you have any questions about our targeted lipidomics services, please contact us.

Case: PCB153-Mediated Disruption of Glycerophospholipid Metabolism in PC12 Cells: A Lipidomics Study


This study focuses on understanding the toxicological effects of PCB153 on glycerophospholipid metabolism in PC12 cells. PCB153, a polychlorinated biphenyl, is known to have adverse health effects, and this research aims to unravel its specific influence on lipid metabolism.


PC12 cells were utilized in the study, obtained from the Chinese Academy of Sciences, Shanghai, China. The exposure to PCB153 occurred at three different concentrations (0.05 μg mL−1, 0.5 μg mL−1, and 20 μg mL−1) over a period of 120 hours.

Technical Methods

Chemicals: High-quality chemicals were employed, including HPLC-MS grade acetonitrile and methanol, HPLC grade 2-propanol and dichloromethane, and HPLC-MS grade ammonium acetate.

Cell Culture: PC12 cells were cultured in RPMI 1640 medium supplemented with fetal bovine serum, penicillin, and streptomycin, maintained at 37 °C with 5% CO2.

Cell Viability Assay: PC12 cells were exposed to different PCB153 concentrations, and viability was measured using the cell counting kit-8 (CCK-8) method at 24-hour intervals up to 120 hours.

Sample Collection and Preparation: Cell samples were collected at various time points, and glycerophospholipids were extracted using a modified method. UHPLC-MS/MS was employed for lipid analysis, and data analysis included statistical methods such as t-tests, PCA, and OPLS-DA.


Method Validation: The study confirmed the presence of 22 glycerophospholipids in PC12 cells. The extraction method was validated for efficiency and stability.

PCB153 Effects on PC12 Cell Viability: Exposure to PCB153 resulted in dosage-dependent decreases in cell viability. Multivariate statistical analysis highlighted the comprehensive impact of PCB153 on lipid metabolism.

Identification of Potential Biomarkers: Five potential glycerophospholipid biomarkers (PC(14:0/14:0), PE(16:0/18:1), PE(16:0/18:2), PS(18:0/18:1), PI(16:0/18:1)) were identified, suggesting their roles in cellular functions and potential implications for neurodegenerative disorders.

Implications and Conclusion: The study suggests a link between PCB153 exposure and glycerophospholipid quantity changes, emphasizing the importance of metabolic disturbances in understanding the toxicity mechanisms. Lipidomics studies could provide valuable insights for risk assessment and neuroprotective therapeutic development.

A scatter plot correlation matrix of the main variables used in the model.The OPLS-DA results among control and dosage groups from 24 h to 120 h

A scatter plot correlation matrix of the main variables used in the model.Heat maps generated by Hierarchical Pearson clustering for 22 target glycerophospholipids after exposure of PCB153 at 96 h (A), 120 h (B)


  1. Wang, Xinlu, et al. "Analysis of glycerophospholipid metabolism after exposure to PCB153 in PC12 cells through targeted lipidomics by UHPLC-MS/MS." Ecotoxicology and environmental safety 169 (2019): 120-127.
* Our services can only be used for research purposes and Not for clinical use.




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