Targeted Lipidomics Service
Lipids are biomolecules that are soluble in non-polar solvents, including fats, sterols, fat-soluble vitamins (such as vitamins A, D, E, and K), monoglycerides, diglycerides, triglycerides, and sheaths Lipids and phospholipids and so on. It is the main component that constitutes the biomembrane, and regulates the signal transduction process of cell growth, differentiation, senescence, and programmed death. It is also involved in physiological functions such as body growth, health, intellectual development, and memory. In addition, it can also affect the occurrence and development of various diseases such as tumor, cardiovascular, endocrine, immune, and nervous system.
When you want to study a specific target lipid or lipid class, you can choose targeted lipidomics. Precise method development allows for the inclusion of equal amounts of isotope-labeled or odd-chain internal standards selected from customizable lipid panels. Lipids from each sample are extracted using standardized protocols and analyzed by GC/LC-MS/MS. Analyte concentrations are determined from calibration curves prepared from authentic standards. This approach is especially useful for quantifying lipids present at low levels in samples, such as endogenous bioactive lipids (e.g., prostaglandins, leukotrienes, or endocannabinoids). Standard statistical analysis tools can reveal changing trends in lipid species among experimental groups, which can be visualized with a heat map or other available graphical tools.
Creative Proteomics provides reliable, fast, and cost-effective targeted lipidomics to accelerate your project.
Our Targeted Lipidomics Services
Liquid chromatography or gas chromatography hyphenated to triple quadrupole mass spectrometers for targeted lipidomics.
- Shimadzu Nexera X2 U-HPLC System and AB Sciex 6500 QTRAP.
- Thermo Scientific TSQ 9000 triple quadrupole GC-MS/MS system.
Fig1. The protocol workflow of targeted lipidomics (Creative Proteomics)
Featured Lipid Analysis Service
Sample Requirements
Samples that can be processed: accept a variety of sample types, including cells, conditioned media, subcellular compartments, exosomes, tissues, blood, plasma, breast milk, skin, urine, feces, plants, microorganisms, and food.
Extraction of lipids should be performed right after sample collection steps, otherwise, samples should be stored at -80°C, and shipped with dry ice. After sample collection, you can either perform sample pretreatment, or contact us for specific sample pretreatment service.
Why Choose Us?
- Creative Proteomics offers one-stop targeted lipidomics services according to customer's detailed requirements.
- Provide a detailed report at the end of the entire project, including the experimental process, MS/MS instrument parameters, quality control parameters, raw data and the final result of the comparison.
If you have any questions about our targeted lipidomics services, please contact us.
* Our services can only be used for research purposes and Not for clinical use.
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