Bile Acids Analysis Service

With extensive experience and state-of-the-art technologies, Creative Proteomics use highly sensitive LC-MS/MS method for the rapid identification and quantification of bile acids, which can satisfy the needs of academic and industrial study.

Overview

Bile Acids Targeted Lipidomics

Bile acids (BA) are major components of bile, synthesized from cholesterol in hepatocytes of the liver, and play key roles in various biological processes (e.g., cholesterol homeostasis, lipid absorption, and production and formation of bile). To obtain a better understanding of the function of BA in these different biological processes and to study their link to a variety of diseases (e.g., hepatobiliary and intestinal dysfunction, obesity), it is important to use fast and reliable analytical methods that are able to qualify and quantify the different BA in various matrices such as plasma, blood, serum, urine, and cell lysates. The observed shifts in the concentration of the total bile acid content or shifts in individual bile acid concentrations can act as important prognostic or diagnostic biomarkers.

Over the last decade, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become the method of choice for the quantification of bile acids (BA) and their conjugates in different matrices, such as plasma, blood, urine, and cell. Currently, a reliable and reproducible method using highly sensitive LC-MS/MS platform for the identification and quantification of BA has been established by the scientists at Creative Proteomics, which can satisfy the needs of academic and industrial study in your lab. The combination of MS instrument and liquid chromatography (LC) can effectively separate target lipid molecules from complex samples.

Advantages of Our Bile Acids Analysis Service

  • The samples that can be analyzed cover a wide range, including cultured cells, conditioned medium, exosomes, tissues, serum, plasma, urine.
  • A detailed report will be provided at the end of the whole project, including the experiment procedure, MS/MS instrument parameter, raw data and data reports with figures, ready to be published.
  • Creative Proteomics designs experimental programs according to customer needs and choose different technical methods to improve detection efficiency and ensure the accuracy and repeatability of experimental results.

The Technical Process of Our Bile Acids Analysis Service

The workflow of bile acids analysis serviceThe workflow of bile acids analysis service

Structural details: species level
Precision: pmol & mol%
Delivery time: 2-4 weeks
Sample requirements
Blood/plasma: 500 ul/sample
Tissue: 200 mg/sample
Cells: 1x107/sample

  • A minimum of 6 replicate plates or wells per condition are required.
  • After the sample is collected, it will be stored at -80°C immediately and transported by dry ice.
  • Tubes should be clearly labeled with sample identifiers and include a hard copy of the list of samples to accompany the shipment. Please include all available sample information when shipping samples.

Deliverables

  • Experimental procedure
  • Parameters of liquid chromatography and MS
  • Purity analysis report
  • MS raw data files and MS data quality checks
  • Hexylceramide quantitative result data
  • Custom analysis report

With years of experience in lipidomics analysis services, Creative Proteomics has a good reputation in supporting the detection and quantification of a variety of lipids. Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, we provide reliable, fast and cost-effective BA analysis services. If you have any questions about our BA targeted lipidomics services, please contact us.

* Our services can only be used for research purposes and Not for clinical use.

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