Isoprostanes Analysis Service

Isoprostanes (IsoPs) are a group of prostaglandin-like compounds that are produced by the reaction of free radicals with arachidonic acid, including arachidonic esters in phospholipids.

IsoPs has been determined to be both biomarkers and mediators of oxidative stress in numerous disease settings.

Creative Proteomics provides comprehensive isoprostanes detection service from lipid extraction, lipid detection and data analysis for biomedical research institutions, biotechnology and pharmaceutical companies.

Isoprostanes Targeted Lipidomics

Our Isoprostane Analysis Service

Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.

High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for isoprostane analysis service (Triple Quad™ 6500 LC-MS/MS System).

The workflow of isoprostanes targeted lipidomicsFig 1. The workflow of isoprostanes targeted lipidomics

Sample Requirements

We routinely cover a rich variety of sample types, including cells, conditioned medium and body fluids such as urine, blood, bile, pericardial and cerebrospinal fluid and lung condensate, in addition to tissues such as vasculature, brain and liver.

Precautions must be taken to prevent their artifactual formation by auto-oxidation during sample processing and storage. Auto-oxidation does not occur in lipid-containing samples, such as plasma or tissue, that are immediately snap-frozen in liquid nitrogen and stored at −80°C for up to eight months.

Why Choose Us?

  • High throughput: Only one injection can run both quantitative MRM and qualitative QTRAP scans to maximize throughput.
  • One-stop analysis service: Provide comprehensive services from sample preparation, standard addition, mass spectrometry analysis, data processing, and data comparison.
  • Fast turnaround time: 1-4 weeks.
  • Provide a detailed report at the end of the entire project, including the experimental process, MS/MS instrument parameters, quality control parameters, raw data and the final result of the comparison.

Applications of Isoprostane Analysis

  • High-quality clinical research
  • Identification of biomarkers
  • Drug development and discovery

If you have any questions about our isoprostanes targeted lipidomics services, please contact us.

Reference:

  1. Praticò D.; et al. The isoprostanes in biology and medicine. Trends in Endocrinology & Metabolism. 2001, 12(6): 243-247.
* Our services can only be used for research purposes and Not for clinical use.

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