Isoprostanes (IsoPs) are a group of prostaglandin-like compounds that are produced by the reaction of free radicals with arachidonic acid, including arachidonic esters in phospholipids.
IsoPs has been determined to be both biomarkers and mediators of oxidative stress in numerous disease settings.
Creative Proteomics provides comprehensive isoprostanes detection service from lipid extraction, lipid detection and data analysis for biomedical research institutions, biotechnology and pharmaceutical companies.
Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.
High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for isoprostane analysis service (Triple Quad™ 6500 LC-MS/MS System).
Fig 1. The workflow of isoprostanes targeted lipidomics
We routinely cover a rich variety of sample types, including cells, conditioned medium and body fluids such as urine, blood, bile, pericardial and cerebrospinal fluid and lung condensate, in addition to tissues such as vasculature, brain and liver.
Precautions must be taken to prevent their artifactual formation by auto-oxidation during sample processing and storage. Auto-oxidation does not occur in lipid-containing samples, such as plasma or tissue, that are immediately snap-frozen in liquid nitrogen and stored at −80°C for up to eight months.
If you have any questions about our isoprostanes targeted lipidomics services, please contact us.