Arachidonic Acid Analysis Service
Arachidonic acid (ARA, AA) is a full cis-5,8.11,14-eicosatetraenoic acid, which is a long-chain polyunsaturated fatty acid of the Omega-6 group. It is a kind of polyunsaturated essential fatty acid with high content and the widest distribution in living organisms. The majority of free arachidonic acid is bound to the second carbon of glycerol in the phospholipids of cell membranes and is released by enzymatic hydrolysis when needed.
Arachidonic acid can be transformed into various metabolites in the body, most of which have strong physiological effects, and a wide range of effects, is an important substance for cell regulation. Arachidonic acid has three main metabolic pathways in living organisms: through cyclooxygenase (COX), lipoxygenase (LOX) or cytochrome P-450 (CYP-450) to produce prostaglandin (PG), thromboxane (TX), leukotiene (LT) or hydroxyfraty acid (OHFA). COXs are divided into 3 forms: COX-1 (constitutive), COX-2 (mitogen-induced form) and COX-3 (variant of COX-1).
Arachidonic acid is released from membrane phospholipids via phospholipase A2s and plays an important biological role in vivo. It is widely involved in immune and inflammatory responses and is a biomarker of inflammation. Accurate qualitative and quantitative analysis of arachidonic acid is of great value in studying the development mechanism, diagnosis and prediction of related diseases.
Creative Proteomics has established an LC-MS assay for arachidonic acid and its metabolites, which allows simultaneous detection of multiple compounds. We are equipped with Thermo Q Exactive and ABsciex Qtrap5500 instruments to enable highly accurate qualitative and quantitative assay services for arachidonic acid and its metabolites.
List of detectable arachidonic acid at Creative Proteomics
| Prostaglandins (PGs) | Prostaglandin H2, Prostaglandin A1, Prostaglandin A2, Prostaglandin D2, Prostaglandin E2, 6-keto-Prostaglandin F1a, Prostaglandin F2a, Prostaglandin I2 |
| Thromboxanes (TBs) | Thromboxane B2 |
| Leukotrienes (LTs) | Leukotriene C4, Leukotriene B4, Leukotriene B5, Leukotriene D4, Leukotriene E4, 6-Trans-leukotriene B4, 11-Trans-leukotriene C4, 12-Trans-leukotriene D4, 13-Trans-leukotriene E4 |
| Hydroxy, dihydroxy arachidonic acids (HETEs) | 5-HETE, 8-HETE, 12-HETE, 15-HETE, 19(R)-HETE, 20-HETE, 5(6)-DiHET, 8(9)-DiHET, (±)11(12)-DiHET, (±)14(15)-DiHET |
| Oxyeicosatrienoic acids (EETs) | 5-oxo-ETE, 5,6-EET, 8,9-EET, 11,12-EET, 14,15-EET |
| Oxyoctadecadienoic acid | 13-OXoODE |
| Hydroxy-octadecadienoic acid | 9-HODE |
| Oxo-octadecadeca-enoic acid | 9,10-EPOME, 12,13-EPOME |
| Dihydroxyoctadecenoic acid | 9,10-DiHoME, 12,13-DiHOME |
Technology platform
Liquid chromatography-mass spectrometry (LC-MS/MS)
A typical workflow of mass spectrometry-based lipidomics (Wang et al., 2019)
Sample requirements
- Serum/Plasma: ≥ 500 ul/sample
- Animal tissue: ≥ 200 mg/sample
- Urine: ≥ 1 ml/sample
- Plant tissue: ≥ 200 mg/sample
- Microorganism ≥ 200 mg/sample
If you want to analyze other samples, please contact our experts for consultation.
Deliverables
- Experimental procedure
- Parameters of liquid chromatography and mass spectrometry
- MS raw data files and MS data quality checks
- Spectrum of fatty acid analysis
- Custom analysis report
Delivery time: 2-4 weeks
Reference:
- Wang, Jianing, Chunyan Wang, and Xianlin Han. "Tutorial on lipidomics." Analytica chimica acta 1061 (2019): 28-41.
* Our services can only be used for research purposes and Not for clinical use.
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