Ergosterol Analysis Service
With extensive experience and state-of-the-art technologies, Creative Proteomics uses highly sensitive LC-ESI/MS method for the rapid identification and quantification of ergosterol in fungi, which can satisfy the needs of academic and industrial study.
Overview
Ergosterol (Erg) is the major sterol present in plasma and mitochondrial membranes of fungi and functions to maintain membrane fluidity, permeability, and structure. Erg also forms lipid rafts, which are enriched with many biologically important molecules such as efflux pumps, sodium and potassium pumps, receptors, and nutrient transporters. These microdomains are central to a variety of cellular processes, stress responses, and adaptations to the environment; maintaining lipid rafts is critical for proper functioning of the cells. It also has multiple functions in growth regulation and is a precursor to vitamin D2. Because of its crucial functions, unique structural properties, and particular biosynthetic steps, ergosterol is the target of the majority of clinically available antifungals.
For the analysis of ergosterol, Creative Proteomics developed a liquid chromatography/mass spectrometry (LC/MS) method to monitor ergosterol biosynthesis. The combination of MS instrument and liquid chromatography (LC) can effectively separate target lipid molecules from complex samples.
Advantages of Our Ergosterol Analysis Service
- Creative Proteomics provides comprehensive services from prepare the samples, run the mass spectrometric analysis, process the data, perform statistical analysis, and identify the compounds.
- Data analysis including PCA, PLS-DA, PLS-R and univariate statistics, we generate a list of lipids that classifies data based on a selection of statistical tests: F-test, t-tests or regression and export lists for further analysis, such as enrichment and KEGG pathway analysis.
- We will use our expertise to help you interpret the results and provide you with actionable recommendations on how to utilize these results in your research or business.
The Technical Process of Our Ergosterol Analysis Service
The workflow of ergosterol analysis service
Structural details: species level
Precision: pmol & mol%
Variants identified: 1
Delivery time: 2-4 weeks
Sample requirements
- 200–350 mg dry weight/sample
- A minimum of 6 replicate plates or wells per condition are required.
- After the sample is collected, it will be stored at -80°C immediately and transported by dry ice.
- Tubes should be clearly labeled with sample identifiers and include a hard copy of the list of samples to accompany the shipment. Please include all available sample information when shipping samples.
Deliverables
- Experimental procedure
- Parameters of liquid chromatography and MS
- Purity analysis report
- MS raw data files and MS data quality checks
- Hexylceramide quantitative result data
- Custom analysis report
With years of experience in lipidomics analysis services, Creative Proteomics has a good reputation in supporting the detection and quantification of a variety of lipids. Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, we provide reliable, fast and cost-effective Erg analysis services. If you have any questions about our Erg targeted lipidomics services, please contact us.
* Our services can only be used for research purposes and Not for clinical use.
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