Creative Proteomics has developed a reliable and reproducible method using highly sensitive LC-MS/MS method for the rapid identification and quantification of Neurofurans (nFs) in different sample types, which can satisfy the needs of academic and industrial study in your lab.

Overview

Neurofurans (nFs) are 22-carbon compounds formed nonenzymatically by free radical mediated peroxidation of docosahexaenoic acid (DHA), an ω-3 essential fatty acid. The neurofurans are similar to the isofurans and are formed under similar conditions of oxidative stress, containing a substituted tetrahydrofuran ring. The fact that nFs are readily detectable in normal brain from animals at concentrations significantly higher than other classes of isoeicosanoids. Some studies demonstrated that the levels of nFs are elevated in the brain cortex of a mouse model of Alzheimer disease. Measurement of the nFs may ultimately prove useful in diagnosis, timing, and selection of dose in the treatment and chemoprevention of neurodegenerative disease.

The combination of MS instrument and liquid chromatography (LC) can effectively separate target lipid molecules from complex samples. High-quality and accurate lipid level analysis helps to study the changes in the content of nFs under physiological and other pathological conditions, and accelerate the analysis of biomarkers and related disease mechanisms.

Why Choose Us?

• Creative Proteomics provides comprehensive services from prepare the samples, run the mass spectrometric analysis, process the data, perform statistical analysis, and identify the compounds.
• Creative Proteomics uses different mass spectrometry methods according to the customer's experimental design.
• Our project is a very complex study that requires a high degree of sensitivity and accuracy. Creative Proteomics has molecular biologists, mass spectrometry specialists, bioinformatics experts, and lipidomics data scientists. We are well-prepared and positioned to foster collaboration in the fast-developing field of lipidomics.

Our Neurofurans Analysis Service

Fig 2. The workflow of neurofurans analysis service.

Structural details: species level
Mode: MRM
Precision: pmol & mol%
Delivery time: 2-4 weeks
Sample requirements
Serum: 100-200 ul/sample
Tissue: 10-50 mg/sample
Cells: 1x10⁷/sample

• After the sample is collected, it will be stored at -80°C immediately and transported by dry ice.
• Tubes should be clearly labeled with sample identifiers and include a hard copy of the list of samples to accompany the shipment. Please include all available sample information when shipping samples.

Deliverables

• Experimental procedure
• Parameters of liquid chromatography and MS
• Purity analysis report
• MS raw data files and MS data quality checks
• Hexylceramide quantitative result data
• Custom analysis report

Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, Creative Proteomics provides reliable, fast and cost-effective neurofurans analysis services. If you have any questions about our neurofurans targeted lipidomics services, please contact us.

Reference:

1. Song, Wen-Liang.; et al. Neurofurans, novel indices of oxidant stress derived from docosahexaenoic acid. Journal of Biological Chemistry. 2008, 283.1: 6-16.

Services:

• Glycerophospholipids Analysis Service
• Glycerolipids Analysis Service
• Sphingolipids Analysis Service
• Fatty Acyls Analysis Service
• Sterol Lipids Analysis Service
• Phospholipids Analysis Service
• Prenol Lipids Analysis Service
• Saccharolipids Analysis Service
• Polyketides Analysis Service
• Untargeted Lipidomics
• Targeted Lipidomics
• Lipidomics Bioinformation Analysis
• Lipidomics Service

Resource:

Platform:

• Mass Spectrometry Systems
• Chromatographic Systems
• Shot-Gun Lipidomics
• GC/LC-MS Lipidomics
• Sample Guidelines