Cells Untargeted Lipidomics
Cells lipidomics approaches utilizing whole cell lysates of cultured cell lines or primary cells support the notion that, like the transcriptome and proteome, the cellular lipidome is actively remolded under various stimuli and physiological conditions.
Identification the lipid profiles of different cells or different states of the same cell can offer novel biomarkers for specific disease, also provide new insights for us to deeply understand the pathogenic mechanism of diseases.
Fig 1. Lipid distribution of animal cells (Lydic et al., 2018)
Creative Proteomics provides comprehensive services from primary cells separation, lipid extraction, lipid detection and data analysis for biomedical research institutions, biotechnology and pharmaceutical companies.
Our Lipidomics Services for Cell Sample
Creative Proteomics is based on the liquid-mass spectrometry (LC-MS) unbiased detection of all lipid molecules in cell samples as much as possible. We use statistical analysis to screen differential lipid molecules, discover the relative relationship between lipid metabolism changes and physiological and pathological changes, and reveal the mechanism of lipids in various life activities.
Fig 2. The workflow of cells untargeted lipidomics.
We also provide other lipid extraction methods according to your experimental needs.
Sample Requirements
| Sample | Sample Requirments | TimeLine |
|---|
| Cell lines | 5x106-1x107 cells | 2-4 weeks |
| Primary cells | 2x106 cells | 4-8 weeks |
We provide different primary cell separation methods according to customer needs, including
- Enzymic hydrolysis
- Immunomagnetic cell separation
- Fluorescence-activated cell sorting (FACS)
- Density gradient centrifugation
- Immunodensity cell isolation
Data Deliverables
a) MS data will be delivered as Excel les with putative identi cation based on the m/z ratio of the analytes.
b) Differential analysis of lipids between treatment groups will be included in the Excel files.
Applications
- Early clinical prediction, diagnosis and treatment research
- Pharmacological research
- Toxicology research
- Phenotype and physiological function research
Why Choose Us?
- Fast turnaround time: 2-4 weeks.
- Professional platform: Cutting-edge apparatuses, reliable data and reproducible process.
- Bioinformatics service: Univariate analysis, multivariate analysis, difference analysis, function analysis, pathway analysis, etc.
- Detailed report: A detailed report to our clients, including experiment procedures and parameters of instruments, MS data with putative identification based on the m/z ratio of the analytes, and customized bioinformatics analysis.
If you have any questions about our lipidomics service, please contact us.
References:
- Lydic, T.A.; Goo, Y. Lipidomics unveils the complexity of the lipidome in metabolic diseases. Clin Trans Med. 2018, 7, 4.
- Gong, J.; et al. Reprogramming of lipid metabolism in cancer-associated fibroblasts potentiates migration of colorectal cancer cells. Cell Death Dis. 2020, 11, 267.
* Our services can only be used for research purposes and Not for clinical use.
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