Leukotrienes (LTs), which are derived from arachidonic acid through two steps catalyzed by 5-lipoxygenase (5-LO), are inflammatory mediators that function in normal host defense and play roles in inflammatory diseases. The LTs are composed of cysteinylleukotrienes (CysLTs) and leukotriene B4 (LTB4).

They exert their biological effects by binding to G-protein-coupled receptors (GPCRs). Different LT receptor subtypes exhibit unique functions and expression patterns.

LT receptors BLT1 and BLT2 are activated by leukotriene B4 (LTB4), whereas CysLT1 and CysLT2 receptors are activated by cysteinyl LTs (CysLTs).

LTs are involved in various inflammatory diseases, including asthma, allergic rhinitis (AR), atopic dermatitis (AD), allergic conjunctivitis, rheumatoid arthritis, anaphylaxis, chronic obstructive pulmonary disease (COPD), obliterative bronchiolitis after lung transplantation, and interstitial lung diseases.

Fig 1. Biosynthetic pathways and receptors for leukotrienes (Jo-Watanabe A et al., 2019)

Creative Proteomics provides comprehensive leukotrienes analysis services from lipid extraction, lipid detection and data analysis for biomedical research institutions, biotechnology and pharmaceutical companies.

Our Leukotrienes Analysis Service

Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.

High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for leukotrienes analysis service (Triple Quad™ 6500 LC-MS/MS System).

Fig 2. The workflow of leukotrienes targeted lipidomics.

Sample Requirements

We routinely cover a rich variety of sample types, including cells, conditioned medium, tissue, serum, plasma, amniotic fluid for our customer.

Extraction of lipids should be performed right after sample collection steps, otherwise, samples should be stored at -80°C, and shipped with dry ice.

After sample collection, you can either perform sample pretreatment, or contact us for specific sample pretreatment service.

Why Choose Us?

• Fast turnaround time: 1-4 weeks.
• High throughput: Only one injection can run both quantitative MRM and qualitative QTRAP scans to maximize throughput.
• One-stop analysis service: Provide comprehensive services from sample preparation, standard addition, mass spectrometry analysis, data processing, and data comparison.
• Bioinformatics analysis: we provide data processing (e.g., data alignment, feature detection), feature prioritization (e.g., principal component analysis, heat maps, ANOVA/t-test, multivariate statistical analysis, self organizing maps, volcano plots, and network activity prediction), and lipid identification services.
• Detailed report: Experimental process, MS/MS instrument parameters, quality control parameters, raw data and the final result of the comparison.

Applications of Leukotrienes Analysis

• Identification potential biomarkers for establishing preventive or therapeutic programs for human diseases.
• Study metabolic pathway mechanisms.

If you have any questions about our leukotrienes targeted lipidomics services, please contact us.

Reference:

1. Jo-Watanabe A et al. The role of leukotrienes as potential therapeutic targets in allergic disorders. International journal of molecular sciences. 2019, 20(14): 3580.

Services:

• Glycerophospholipids Analysis Service
• Glycerolipids Analysis Service
• Sphingolipids Analysis Service
• Fatty Acyls Analysis Service
• Sterol Lipids Analysis Service
• Phospholipids Analysis Service
• Prenol Lipids Analysis Service
• Saccharolipids Analysis Service
• Polyketides Analysis Service
• Untargeted Lipidomics
• Targeted Lipidomics
• Lipidomics Bioinformation Analysis
• Lipidomics Service

Resource:

Platform:

• Mass Spectrometry Systems
• Chromatographic Systems
• Shot-Gun Lipidomics
• GC/LC-MS Lipidomics
• Sample Guidelines