Glycerolipids and their precursors are synthesized and metabolized by enzymes related to cytoplasm and membrane organelles (including endoplasmic reticulum, mitochondria and lipid droplets). The esterification of one, two or three fatty acyl groups with glycerol produces mono-, di- and tri-substituted glycerides called monoacylglycerol (MAG), diacylglycerol (DAG) and triacylglycerol (TAG). Glycerolipids are a group of structurally heterogeneous lipids that play key structural and functional roles in bacterial, plant and animal membranes. Glycerolipids also play an important role in cell signal transduction, membrane transport and membrane protein anchoring.
Creative Proteomics provides rapid and cost-effective glycerolipids analysis based on a highly stable, repeatable, and highly sensitive system for separation, characterization, identification, and quantitative analysis.
Fig1. Glycerolipids: structures and metabolic steps involved (Xicoy H.; et al, 2019)
Glycerolipids analysis is conducted on GC/MS, LC/MS platforms at Creative Proteomics. We have pioneered the use of contemporary chromatography systems and high resolution mass spectrometers to identify and characterize several lipid classes.
Fig2. The workflow of glycerolipids analysis service
We can accept a variety of sample types, including cells, tissues, blood, plasma, skin, urine, feces, plants, microorganisms, et al.
Extraction of lipids should be performed right after sample collection steps, otherwise, samples should be stored at -80°C, and shipped with dry ice. After sample collection, you can either perform sample pretreatment, or contact us for specific sample pretreatment service.
If you have any questions about our glycerolipids analysis services, please contact us.