Sphingolipids and their metabolizing enzymes are key mediators in vivo. Sphingosine kinases (SphKs) and their lipid product sphingosine 1-phosphate (S1P) play an important role in signaling and in the development and progression of diseases, especially in chronic inflammatory and autoimmune diseases. S1P is a sphingolipid metabolite with multiple biological activities and is widely present in platelets, erythrocytes, neutrophils and lymphatic fluid, blood and other cells and body fluids.S1P is generated and can act intra- and extracellularly. On the one hand, it acts on cell surface receptors through autocrine and paracrine modes to exert its extracellular effects, including cell proliferation and migration, inflammatory cytokine infiltration, angiogenesis, and autoimmunity. On the other hand, it functions as an intracellular second messenger and acts directly on intracellular targets to regulate Ca2+ levels, gene transcription, protein modification, etc.
Fig 1. Intracellular and extracellular actions of S1P
To facilitate the drug discovery of S1P signaling pathway and the mechanism of S1P involvement in various diseases, Creative Proteomics has developed a reliable and reproducible method for rapid identification and quantification of S1P in different sample types using a highly sensitive LC-MS/MS method that can meet the needs of your laboratory academic and industrial research.
Creative proteomics uses a liquid chromatography triple quadrupole mass spectrometry analysis system (QTRAP 4500 LC-MS/MS System) to achieve qualitative and quantitative analysis of sphingosine 1-phosphate.
Fig 2. The workflow of sphingosine 1-phosphate analysis service
We can accept a variety of sample types, including cells, conditioned medium, subcellular compartments, exsome, tissue, blood, plasma, skinfor our customer.
After sample collection, you can either perform sample pretreatment, or contact us for specific sample pretreatment service.
If you have any questions about our sphingosine 1-phosphate analysis services, please contact us.