Thromboxane (TX) is named for its role in clot formation. The two major thromboxanes are thromboxane A2 and thromboxane B2. Thromboxane A2 (TXA2) is active but is very unstable and has a half-life of only 30 seconds before it undergoes hydrolysis to form thromboxane B2 (TXB2) which is inactive.
TXA2 was originally described as being released from platelets and is now known to be released by a variety of other cells including macrophages, neutrophils, and endothelial cells. Named after its role in thrombosis, TXA2 has prothrombotic properties, as it stimulates the activation of platelets and platelet aggregation. TXA2 is also a known vasoconstrictor and gets activated during times of tissue injury and inflammation. While the prostaglandin counterbalances its thrombotic and vasoconstrictor properties prostacyclin (PGI2), there are various physiological and pathological situations where this balanced becomes dysregulated. Increased activity of TXA2 may play a role in the pathogenesis of myocardial infarction, stroke, atherosclerosis, and bronchial asthma. Increased action of TXA2 also has implications in pulmonary hypertension, kidney injury, hepatic injury, allergies, angiogenesis, and metastasis of cancer cells.
Fig 1. Thromboxane biosynthesis pathway (Ghoshal K et al., 2014)
Creative Proteomics provides comprehensive thromboxanes targeted lipidomics services from lipid extraction, lipid detection and data analysis for biomedical research institutions, biotechnology and pharmaceutical companies.
Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.
High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for thromboxanes analysis service (Triple Quad™ 6500 LC-MS/MS System).
Fig 2. The workflow of thromboxanes analysis service.
We receive a variety of samples, including but not limited to plasma,cell, tissue.
After sample collection, you can either perform sample pretreatment, or contact us for specific sample pretreatment service.
If you have any questions about our thromboxanes detection service, please contact us.