The origin of a disease and the origin of metabolic changes—often lies in the cellular processes of the tissue. To characterize metabolic changes underlying a disease phenotype, it is advantageous to analyze metabolite concentrations in tissue samples of animal models or human biopsy samples. Lipids are an important component of tissues, so studying lipid metabolism changes in tissues can can provide unique information on physiological or pathological mechanisms elucidation and biomarker discovery.
Creative Proteomics provides comprehensive tissues untargeted lipidomics services from lipid extraction, lipid detection and data analysis for scientific researchers, biomedical research institutions, biotechnology and pharmaceutical companies.
Creative Proteomics is based on the liquid-mass spectrometry (LC-MS) unbiased detection of all lipid molecules in animal tissue samples as much as possible. The experimental group and the control group are compared and analyzed. Through statistical analysis to screen differential lipid molecules, and then to find the relative relationship between lipid metabolism changes and physiological and pathological changes, and reveal the mechanism of lipids in various life activities.
Fig 1. The workflow of tissues untargeted lipidomics.
For Tissue, the amount used should be empirically determined beforehand based on tissue type used. We usually need 10-50 mg tissue for our untargeted lipidomics services.