Ceramide (CER) is a sphingosine lipid composed of sphingosine long-chain bases and fatty acids. Ceramide is the central hub of the sphingolipid pathway, including sphingomyelin, glucosylceramide and galactosylceramide. It is mainly divided into hexose ceramide (HexCer), lactose ceramide (LacCer), sphingosine (Sph), dihydro sphingosine (DhSph) and dihydroceramide (DhCer).
Ceramide has a strong ability to associate with water molecules. It maintains skin moisture by forming a network structure in the stratum corneum. In addition, it also plays an important role in maintaining the integrity of cell membranes, cell stress response, inflammatory signal transduction and cell apoptosis.
Creative Proteomics uses liquid chromatography mass spectrometry (LC-MS) method to identify the samples, which can effectively and accurately detect the changes in the content of ceramide.
Creative Proteomics use liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) for ceramide detection.
Fig 1. The workflow of ceramides analysis service.
|Hexose Ceramide (HexCer)||Lactose Ceramide (LacCer)||Sphingosine (Sph)|
|Dihydrosphingosine (DhSph)||Dihydroceramide (DhCer)||Cer(d14:1/16:0)|
We can accept a variety of sample types, including cells, conditioned medium, subcellular compartments, exsome, tissue, blood, plasma, skinfor our customer.
After sample collection, you can either perform sample pretreatment, or contact us for specific sample pretreatment service.
If you have any questions about our ceramides analysis services, please contact us.