Ceramides Analysis Service

Ceramide (CER) is a sphingosine lipid composed of sphingosine long-chain bases and fatty acids. Ceramide is the central hub of the sphingolipid pathway, including sphingomyelin, glucosylceramide and galactosylceramide. It is mainly divided into hexose ceramide (HexCer), lactose ceramide (LacCer), sphingosine (Sph), dihydro sphingosine (DhSph) and dihydroceramide (DhCer).

Ceramide has a strong ability to associate with water molecules. It maintains skin moisture by forming a network structure in the stratum corneum. In addition, it also plays an important role in maintaining the integrity of cell membranes, cell stress response, inflammatory signal transduction and cell apoptosis.

Creative Proteomics uses liquid chromatography mass spectrometry (LC-MS) method to identify the samples, which can effectively and accurately detect the changes in the content of ceramide.

Our Ceramides Analysis Service

Creative Proteomics use liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) for ceramide detection.

  • QTRAP 4500 LC-MS/MS System

The workflow of ceramides analysis service.Fig 1. The workflow of ceramides analysis service.

Our Detectable Ceramides Include But Are Not Limited To

Hexose Ceramide (HexCer)Lactose Ceramide (LacCer)Sphingosine (Sph)
Dihydrosphingosine (DhSph)Dihydroceramide (DhCer)Cer(d14:1/16:0)
Cer(d14:1/22:0)Cer(d15:1/20:0)Cer(d15:1/22:0)
Cer(d16:1/17:0)Cer(d16:1/23:0)Cer(d18:1/14:0)
Cer(d18:0/15:0)Cer(d 18:0/16:0)Cer(d18:0/18:0)
Cer(d18:1/20:0)Cer(d18:1/22:0)Cer(d18:0/24:0)
Cer(d18:0/24:1)Cer(d18:0/26:0)Cer(d18:0/26:1)
Cer(d18:0/26:2)Cer(d18:2/23:0) 

Sample Preparation

We can accept a variety of sample types, including cells, conditioned medium, subcellular compartments, exsome, tissue, blood, plasma, skinfor our customer.
After sample collection, you can either perform sample pretreatment, or contact us for specific sample pretreatment service.

Applications

  • Ceramide qualitative and quantitative detection
  • Identification potential biomarkers
  • Study metabolic pathway mechanism

Why Choose Us?

  • One-stop analysis service: Provide comprehensive services from sample preparation, standard addition, mass spectrometry analysis, data processing, and data comparison.
  • High sensitivity: High-sensitivity mass spectrometer is used to effectively detect low-level metabolites in the sample.
  • Fast turnaround time: 1-4 weeks.
  • Detailed report: Provide a detailed report at the end of the entire project, including the experimental process, MS/MS instrument parameters, quality control parameters, raw data and the final result of the comparison.

If you have any questions about our ceramides analysis services, please contact us.

* Our services can only be used for research purposes and Not for clinical use.

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