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Ceramide Quantification Analysis

Ceramide quantification provide accurate, reliable data to help clients analyze ceramide profiles, discover biomarkers, and understand lipid-mediated pathways, supporting research in fields like skincare, drug development, and disease study.

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  • Service We Provide
  • List of Ceramides
  • Methods
  • Advantages
  • Deliverables and Data Analysis
  • Sample Requirements
  • FAQ
  • Publication

What are Ceramides?

Ceramides are lipid molecules that belong to the sphingolipid family and play essential roles in cellular structure and function. They consist of a sphingoid base (such as sphingosine or sphinganine) linked to a fatty acid. Ceramides are crucial for maintaining the integrity of cell membranes, regulating signaling pathways, and influencing processes such as apoptosis, cell growth, and differentiation. In humans, ceramides are particularly abundant in the skin, where they help maintain the skin's barrier and hydration. Additionally, ceramides act as second messengers in cellular signaling, affecting lipid raft properties and cellular responses to stress. Their role in both health and disease underscores the importance of accurate ceramide quantification in biological research.

Ceramide Service in Creative Proteomics

At Creative Proteomics, we offer comprehensive fatty acid analysis services tailored to meet the unique needs of researchers, clinicians, and industry professionals. Our services cover a wide range of fatty acid profiles and molecular data to facilitate in-depth analysis.

Ceramides Profiling

We offer comprehensive profiling of ceramide species across various biological samples, including plasma, tissues, and cell cultures.

Ceramide Biomarker Discovery

Through advanced lipidomics technologies, we assist in discovering novel ceramide-related biomarkers for disease diagnosis, prognosis, and therapeutic monitoring.

Pathway Analysis

Our analysis provides insight into ceramide-mediated signaling pathways and their involvement in cellular processes such as apoptosis, inflammation, and cellular stress responses.

Structural Characterization

We utilize high-resolution mass spectrometry to identify and characterize ceramide species, including variations in fatty acid composition and sphingoid base structure.

Quantitative Analysis  

Accurate and reliable quantification of ceramide species in complex biological matrices, presented in both pmol and mol% concentrations.

List of Detected Ceramides

Ceramide SpeciesDescription
Ceramide C16:0Palmitoyl ceramide (C16:0) – A common ceramide found in the skin.
Ceramide C18:0Stearoyl ceramide (C18:0) – Involved in cell membrane structure.
Ceramide C18:1Oleoyl ceramide (C18:1) – Plays a role in skin hydration and barrier function.
Ceramide C20:0A fatty acid chain of 20 carbon atoms, commonly detected in various tissues.
Ceramide C24:0Lignoceroyl ceramide (C24:0) – Typically found in neural tissues.
Ceramide 1-phosphateA phosphorylated form of ceramide involved in cell signaling.
α-Hydroxyceramide (ACer)Ceramide with an α-hydroxy fatty acid, relevant for various skin conditions.
Ceramide d18:1/18:0A sphingoid base ceramide with a combination of sphingosine and stearic acid.
Ceramide d18:1/24:0A sphingoid base ceramide with lignoceric acid, important in neuronal tissues.

What Methods are Used for Our Ceramides Analysis?

Our primary method for ceramide analysis involves high-resolution mass spectrometry (HRMS), specifically utilizing the Q Exactive Orbitrap system.

Instrumentation

Q Exactive Orbitrap: This mass spectrometer offers an exceptional resolution of up to 280,000, providing detailed information about ceramide species even in complex biological samples. The system is equipped with both positive and negative ion modes, allowing for comprehensive lipidomics analysis.

Analysis Techniques

  • Untargeted Lipidomics: We perform an untargeted analysis of ceramides, enabling the discovery of novel ceramide species and pathways without predefined assumptions. This approach is ideal for exploratory research.
  • Targeted Lipidomics: For more focused research, we provide targeted quantification of specific ceramide species, offering highly precise results for known ceramide targets.
  • Liquid Chromatography-Mass Spectrometry (LC-MS/MS): Our combination of LC with mass spectrometry ensures efficient separation of ceramide species before identification and quantification, enhancing the sensitivity and specificity of the analysis.
Thermo Fisher Q Exactive

Thermo Fisher Q Exactive (Figure from Thermo Fisher)

Why Choose Our Ceramides Services?

  • High Sensitivity and Precision: Detect ceramides at concentrations as low as 0.1 pmol. Achieve ±5% accuracy in ceramide quantification.
  • Comprehensive Profiling: Detect 50+ ceramide species, including rare and modified forms. Identify ceramides with varying sphingoid bases and fatty acid chains (C14 to C24+).
  • Fast Turnaround: Results within 2-6 weeks, with 95% completed on time.
  • Advanced Technology: Achieve 280,000 resolution, ensuring accurate detection of isomeric ceramides. Signal-to-noise ratio of >1000 for major ceramides.
  • Customization and Flexibility: 90-95% specificity for targeted analysis and 100% discovery potential for untargeted. Tailored analysis for specific research needs.
  • Quality Control: Ensure consistency with <2% variation using spiked internal standards. Verified through repeat analyses for accuracy.

Ceramides Analysis Service: Deliverables and Data Analysis

Lipidome Data Analysis WorkflowWorkflow of Lipidome Data Analysis

Creative Proteomics provides a comprehensive ceramide analysis with clear, actionable results:

  • Quantitative Data: Concentrations of all detected ceramide species (pmol, mol%).
  • Principal Component Analysis (PCA): Visual grouping of samples to highlight similarities and differences.
  • Extended Profiles: Chain length, saturation, and hydroxylation for each ceramide class.

For deeper insights, we also provide:

  • Statistical Analysis: ANOVA to detect significant differences across groups.

This all-in-one report ensures you get the key data and insights for your ceramide research.

NPLC-APCI-MS total ion current of (A) equimolar (250 fmol/CER) synthetic human CER mixture containing CER [EOS], [NS], [EOP], [NP], [AS], and [AP]; (B) crude lipid extract of human SC

NPLC-APCI-MS total ion current.
(Figure from van Smeden, Journal of lipid research 52.6, 2011)

MS/MS spectra of synthetic CERs using an ion trap (IT) system: (A) CER [N(18)S(24)]; (B) CER [N(18)dS(24)]; (C) CER E(18:2)O(30)S(18)]; (D) human SC CER subclass [EOS] C70, parent m/z 1050.9 amu; (E) unknown human SC CER, parent m/z 1052.9 amu.

MS/MS spectra of synthetic CERs using an ion trap (IT) system
(Figure from van Smeden, Journal of lipid research 52.6, 2011)

MS/MS spectrum of ceramide N-(tetracosanoyl)-dihydroceramide, with characteristic fragment ions highlighted

MS/MS spectrum of ceramide N-(tetracosanoyl)-dihydroceramide
(Figure from Cornellison, International Journal of Trichology, 2011)

Explore our Lipidomics Solutions brochure to learn more about our comprehensive lipidomics analysis platform.

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What Our Ceramides Analysis Used For

Cosmetic Industry

Ceramide quantification supports the development of skincare products targeting barrier repair, anti-aging, and hydration, helping brands optimize formulations for various skin needs.

Pharmaceutical Research

Evaluating ceramide-targeting drugs in cancer, neurodegenerative diseases, and metabolic disorders.

Biotechnology and Synthetic Biology

Supporting metabolic engineering, gene therapy, and synthetic biology by modulating lipid metabolism.

Neuroscience

Studying neurodegenerative diseases like Alzheimer's and Parkinson's by investigating their role in neuroinflammation and cell death.

Environmental Toxicology

Assessing cellular responses to environmental toxins and pollutants.

Agricultural and Plant Science

Ceramide profiling enhances understanding of plant stress responses, improving crop resilience and stress tolerance.

Sample Requirements for Ceramides Quantification Analysis

Sample TypeRequirementVolume/AmountCondition
Biological SampleTissue, blood, plasma, or cell culture50-100 mg tissue / 200-500 µL plasmaFresh or frozen, stored at -80°C
Skin SamplesSkin biopsies or stratum corneum50-100 mgFresh or frozen, stored at -80°C
Cell LysatesCells harvested from culture1-10 million cellsStored at -80°C or immediately processed
Plant SamplesLeaves, stems, roots50-100 mgFresh or frozen, stored at -80°C
Environmental SamplesEnvironmental tissue (e.g., soil, plant matter)50-100 mgFresh or frozen, stored at -80°C
Extracted LipidsLipid extract in appropriate solvent (e.g., chloroform, methanol)50-100 µL lipid extractStored at -20°C or -80°C

FAQs for Ceramides Quantification Analysis

What is the sensitivity of ceramide quantification using your methods?

Our ceramide quantification services are highly sensitive, with the ability to detect ceramide species at concentrations as low as 0.1 pmol. This ensures accurate measurements even in complex biological matrices, where ceramides are present at trace levels. Our high-resolution mass spectrometry ensures the precise identification and quantification of ceramides, minimizing the risk of false positives or missed species.

Can I analyze both common and rare ceramide species?

Yes, our analysis can detect both common and rare ceramide species, including those with modifications such as hydroxylation or unusual fatty acid chains. We cover over 50 ceramide species, from the most abundant ones like C16:0 and C18:0 to rarer forms such as ceramide C24:0 and α-hydroxyceramides. Our untargeted lipidomics approach allows for comprehensive profiling, including the identification of novel ceramides.

How do you ensure the accuracy and reliability of the ceramide quantification results?

We employ rigorous quality control (QC) measures at each step of the analysis process. All samples undergo internal standard spiking to monitor extraction efficiency and minimize sample variation. The results are cross-checked with multiple analyses for consistency. Additionally, we use high-resolution mass spectrometry with a signal-to-noise ratio greater than 1000, ensuring precise and reproducible quantification.

How long does it take to receive the results from ceramide quantification analysis?

The turnaround time for ceramide quantification analysis is typically between 2 to 6 weeks, depending on the complexity and scope of the analysis. Most projects are completed within this time frame, with 95% of results delivered on time. We provide regular updates throughout the process to keep you informed of the progress.

Can you quantify ceramides in different matrices like skin, plasma, and tissues simultaneously?

Yes, we are capable of analyzing ceramide levels in multiple types of biological matrices within a single study. We can analyze samples such as plasma, tissues, and skin biopsies concurrently, providing a comprehensive overview of ceramide distribution and levels across different biological compartments. This is particularly useful for comparative studies or research on ceramide-related pathologies.

Publications

References

  1. van Smeden, Jeroen, et al. "LC/MS analysis of stratum corneum lipids: ceramide profiling and discovery [S]." Journal of lipid research 52.6 (2011): 1211-1221.
  2. Cornellison, C. D., et al. "MALDI-MS redox lipidomics applied to human hair: A first look." International Journal of Trichology 3.1 (2011): 25-27.
* Our services can only be used for research purposes and Not for clinical use.

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