Cholesteryl Ester Analysis Service
Cholesteryl esters are a dietary lipid, which derived from cholesterol. Cholesteryl esters are formed when the body produces enzymes to produce a metabolic reaction with the cholesterol itself. The ester bond is formed between the carboxylate group of a fatty acid and the hydroxyl group of cholesterol.
Cholesteryl esters are largely spread in body fluids of animals (plasma lipoproteins) and may be also found in vessel walls as fatty streaks in atherosclerosis. Cholesteryl esters have a lower solubility in water due to their increased hydrophobicity.
Creative Proteomics provides you with efficient and accurate qualitative and quantitative detection services of cholesteryl ester through advanced chromatography and mass spectrometry platforms.
Our Cholesteryl Ester Analysis Service
Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.
High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for cholesteryl esters analysis service (Triple Quad™ 6500 LC-MS/MS System).
Fig 2. The workflow of cholesteryl esters analysis service
Sample Requirements
Creative Proteomics has rich experience in cholesteryl esters targeted lipidomics. We routinely cover a rich variety of sample types, including cells, conditioned medium, tissue, blood, plasma.
| Cell | 1*107 |
| Cell Medium | 2 ml |
| Serum | 0.5-1 ml |
| Plasma | 0.5-1 ml |
| Tissue | 5-10 mg |
| Other | Inquire |
Why Choose Us?
- With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective cholesteryl ester analysis services.
- Creative Proteomics establishes different experimental schemes according to customer needs and provide one-stop service from lipid extraction, lipid detection and data analysis, which only takes 2-4 weeks
- Tailor extraction protocols and derection methods to target different needs of customer.
- Creative Proteomics full-service facility provides data processing (e.g., data alignment, feature detection), feature prioritization (e.g., principal component analysis, heat maps, ANOVA/t-test, multivariate statistical analysis, self organizing maps, volcano plots, and network activity prediction), and lipid identification services.
If you have any questions about our cholesteryl esters analysis services, please contact us.
Reference:
- Burdge G C.; et al. A method for separation of phosphatidylcholine, triacylglycerol, non-esterified fatty acids and cholesteryl esters from plasma by solid-phase extraction. British Journal of Nutrition. 2000, 84(5): 781-787.
* Our services can only be used for research purposes and Not for clinical use.
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