Cholesterol Analysis Service

Cholesterol is a lipid-like compound that is a derivative of cyclopentane polyhydrophenanthrene. Cholesterol is widely found in animals, especially in brain and nerve tissues, and is more abundant in kidney, spleen, skin, liver and bile.

The basic biological function of cholesterol is to regulate the physicochemical properties of cell membranes and the conformation of membrane proteins. In addition, cholesterol is an essential synthetic precursor for bile acids and sterol hormones and covalently modifies Hedgehog and its downstream Smoothened proteins. Cholesterol metabolic homeostasis is essential for the maintenance of cellular and organismal life activities. Abnormal cholesterol metabolism is closely related to the development of cardiovascular diseases, neurodegenerative diseases and tumors.

Therefore, identification the cholesterol level can offer novel biomarkers for specific disease, also provide new insights for us to deeply understand the pathogenic mechanism of diseases. Creative Proteomics provides you with efficient and accurate qualitative and quantitative detection services of cholesterol through advanced chromatography and mass spectrometry platforms.

Our Cholesteryl Analysis Service

Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.

High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for cholesteryl analysis service (Triple Quad™ 6500 LC-MS/MS System).

The workflow of cholesteryl analysis serviceFig 1. The workflow of cholesteryl analysis service

Sample Requirements

We can accept many types of samples, including but not limited to: Serum, plasma, urine, bile, cholic acid, cells, liver, brain tissue and other animal tissues and feces, plants, yeasts, and microorganisms, etc.

  • Blood samples, bile ≥ 10 microliters
  • Various tissues ≥ 10 mg
  • Stool ≥ 10 mg

For other sample types and dosage, please contact us.

Please use a sufficient amount of dry ice for transportation and seal it with a foam box. Minimize the possibility of sample degradation during transportation.

Why Choose Us?

  • Fast turnaround time: 1-4 weeks.
  • High throughput: Only one injection can run both quantitative MRM and qualitative QTRAP scans to maximize throughput.
  • One-stop analysis service: Provide comprehensive services from sample preparation, standard addition, mass spectrometry analysis, data processing, and data comparison.
  • Detailed report: Experimental process, MS/MS instrument parameters, quality control parameters, raw data and the final result of the comparison.
  • We will use our expertise to help you interpret the results and provide you with actionable recommendations on how to utilize these results in your research or business.

If you have any questions about our cholesterol analysis services, please contact us.

Reference:

  1. Kuzu, Omer F et al. The role of cholesterol in cancer. Cancer research. 2016, 76.8: 2063-2070.
* Our services can only be used for research purposes and Not for clinical use.

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