Mitochondria Lipid Metabolism Analysis - Lipidomics|Creative Proteomics

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Mitochondria Lipid Metabolism Analysis

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What is Mitochondria Lipid Metabolism?

Mitochondria, the central hubs of energy production and metabolic regulation, are deeply reliant on their lipid environment for structural integrity and functional efficacy. Lipid metabolism within mitochondria is a highly regulated process that underpins bioenergetic pathways, membrane dynamics, signaling cascades, and apoptotic processes.

Why Analyze Mitochondria Lipid Metabolism?

Understanding mitochondrial lipid composition and metabolism is fundamental to:

Deciphering metabolic phenotypes in cellular and subcellular systems
Profiling lipid remodeling under varying nutritional, stress, or pharmacological conditions
Mapping mitochondrial membrane dynamics, signaling roles, and lipid-protein interactions
Supporting systems biology and metabolic modeling through lipidomic flux data
Optimizing bioenergetic performance in biotechnological and bioengineering contexts

Our service offers unmatched analytical depth and specificity across mitochondrial lipid classes, ensuring researchers gain mechanistic clarity from high-precision data.

Mitochondria Lipid Metabolism Analysis Service in Creative Proteomics

Creative Proteomics provides a comprehensive suite of service items tailored to dissect the complexity of mitochondrial lipid metabolism with high specificity and reproducibility. Each module is designed to target key lipid classes, metabolic intermediates, and dynamic processes involved in mitochondrial lipid homeostasis.

Quantitative Profiling of Mitochondrial Lipid Classes

Absolute and relative quantification of key mitochondrial lipid species (e.g., CL, PE, PG, PC, LPC) via internal standard-based LC-MS/MS.

Mitochondrial Fatty Acid β-Oxidation Intermediate Analysis

Measurement of acyl-carnitines and β-oxidation intermediates to assess mitochondrial energy metabolism using targeted MRM analysis.

Cardiolipin Remodeling Assessment

High-resolution Q-TOF and LIPID MAPS® database integration enable identification of oxidized lipids (e.g., 4-HNE), acetylated/glycosylated phospholipids, and branched-chain fatty acids, elucidating roles in oxidative stress and signaling pathways.

Reactive Lipid Species Quantification

Sensitive detection of lipid peroxidation byproducts such as 4-HNE and MDA—biomarkers of mitochondrial oxidative stress.

Isotope Tracer-Based Lipid Metabolism Flux Analysis

Use of ¹³C or ²H-labeled substrates to trace lipid synthesis, degradation, and turnover rates within mitochondrial pathways.

Subcellular Lipid Enrichment

Isolation of mitochondrial fractions followed by lipidomics to ensure subcellular specificity and exclude cytosolic interference.

Lipid Pathway Enrichment and Bioinformatics Analysis

Bioinformatic analysis including PCA, volcano plots, KEGG/LIPID MAPS pathway mapping, and metabolite set enrichment.

Custom Lipid Panel Development

Tailored method development for detection of niche or novel mitochondrial lipids based on your specific project goals.

Detected Lipids and Pathway-Associated Metabolites

Lipid/Metabolite Class	Representative Molecules	Associated Metabolic Pathways
Cardiolipins (CL)	CL(72:8), CL(70:5), CL(68:4)	Mitochondrial membrane integrity, cardiolipin remodeling
Monolysocardiolipins (MLCL)	MLCL(54:4), MLCL(56:3)	Tafazzin-mediated CL remodeling, Barth syndrome pathway
Phosphatidylethanolamines (PE)	PE(38:6), PE(36:2), PE(40:4)	Phospholipid biosynthesis, mitochondrial fusion/fission
Phosphatidylglycerols (PG)	PG(36:2), PG(38:4)	CL precursor biosynthesis, mitochondrial phospholipid pool
Phosphatidylcholines (PC)	PC(34:1), PC(36:4), PC(40:6)	PC-to-PE conversion, mitochondrial lipid exchange
Lysophospholipids (LPC, LPE)	LPC(18:1), LPE(20:4)	Phospholipid turnover, membrane remodeling
Acylcarnitines	C2:0, C8:0, C16:0, C18:1	Fatty acid β-oxidation, carnitine shuttle
Fatty Acids	Palmitate (C16:0), Oleate (C18:1), Linoleate (C18:2)	β-oxidation, phospholipid acyl chain pool
Lipid Peroxidation Products	4-HNE, MDA, isoprostanes	Oxidative stress, mitochondrial membrane damage
Isoprenoids/Coenzymes	CoQ10 (ubiquinone), CoQ9	Electron transport chain, ROS regulation
Glycerolipids	DAG(36:2), TAG(54:3)	Lipid storage, mitochondrial lipid trafficking
Sphingolipids	Ceramide, sphingosine, SM(d18:1/16:0)	Apoptotic signaling, mitochondrial outer membrane integrity
Mitochondrial Sterols	Cholesterol, 7-dehydrocholesterol	Membrane fluidity, steroid biosynthesis interface

Why Choose Our Mitochondria Lipidomics Services?

Ultra-sensitive detection (<1 pmol): Enables precise quantification of low-abundance mitochondrial lipids, even from limited or rare samples.
Extensive lipid coverage: Profiles over 300 mitochondrial lipid species, including cardiolipins, acyl-carnitines, and remodeling intermediates.
Comprehensive pathway resolution: Data mapped to 40+ mitochondrial metabolic and signaling pathways via KEGG and LIPID MAPS.
High quantification accuracy (CV < 10%): Internal standard calibration ensures reproducibility across biological replicates.
Dual-mode analysis (untargeted + targeted): Combines high-resolution Orbitrap profiling with precise MRM quantification.

Advanced instrumentation platform: Powered by Thermo Q Exactive™ HF and SCIEX Triple Quad™ 6500+ for superior resolution and dynamic range.
Subcellular specificity: Optional mitochondrial isolation service ensures lipid origin is strictly organelle-specific.
Stable isotope flux support: Enables ¹³C- or ²H-labeled tracer analysis for dynamic metabolic studies.
Advanced bioinformatics included: PCA, volcano plots, lipid clustering, and pathway enrichment all standard in reporting.
Customizable analysis modules: Tailored lipid panels and method development available based on project goals.

How Creative Proteomics Provides Mitochondria Lipid Metabolism Assay

Workflow for Mitochondria Lipid Metabolism Analysis

What Platforms are Used for Our Mitochondria Lipid Metabolism Analysis?

AB SCIEX 6500+ QTRAP (Figure from SCIEX)

Thermo Fisher Q Exactive (Figure from Thermo Fisher)

Agilent 1260 Infinity II HPLC (Figure from Agilent)

Mitochondria Lipid Metabolism Analysis Service: Results and Data Analysis

Raw Data Files: Complete LC-MS/MS datasets (Thermo .raw and SCIEX .wiff formats).

Processed Data Tables: Quantified lipid species with retention time, m/z, fold change, and statistical significance.

Lipid Identification Report: Matched IDs using LIPID MAPS and HMDB, including lipid class, fatty acyl composition, and adducts.

Internal Standard Normalization: Fully corrected intensity values for reliable comparisons.

Multivariate Statistical Outputs:

Principal Component Analysis (PCA)
Hierarchical clustering heatmaps
Volcano plots for differential lipid expression

Pathway Enrichment Results: Mapped mitochondrial lipid-related pathways via KEGG or Reactome.

Graphical Summary: Custom plots highlighting key altered lipid species and pathways..

Interpretation Notes: Expert-curated biological insights based on your experimental context

Volcano Plot

PCA Analysis

Heatmap

Explore our Lipidomics Solutions brochure to learn more about our comprehensive lipidomics analysis platform.

Download Brochure

Sample Requirements for Mitochondria Lipidomics Solutions

Sample Type	Minimum Amount Required	Notes
Cultured Cells	≥ 1 × 10⁷ cells	Fresh or frozen; recommend gentle homogenization for mitochondrial purity
Tissue Samples	≥ 50 mg	Flash frozen in liquid nitrogen; avoid freeze-thaw cycles
Isolated Mitochondria	≥ 100 µg total mitochondrial protein	Purified by differential or density gradient centrifugation
Biofluids (e.g., plasma)*	≥ 200 µL	*For studies involving mitochondrial-derived vesicles or indirect profiling
Storage Condition	–80°C	Ship on dry ice to preserve lipid integrity
Replicates	≥ 3 biological replicates	Ensures statistical confidence and reproducibility

What Our Mitochondria Lipidomics Analysis Is Used For

Metabolic Research

Investigating mitochondrial lipid remodeling in energy metabolism and nutrient sensing.

Neuroscience

Exploring mitochondrial lipid dynamics in neurodegeneration and synaptic plasticity.

Aging Studies

Profiling lipid changes linked to mitochondrial dysfunction in age-associated decline.

Mitochondrial Bioenergetics

Evaluating lipid compositions that affect membrane potential and oxidative phosphorylation.

Toxicology

Assessing lipid peroxidation and mitochondrial damage in xenobiotic or drug exposure studies.

Cell Stress Response

Monitoring lipid alterations under oxidative stress, hypoxia, or nutrient deprivation.

FAQs for Mitochondria Lipidomics Analysis Service

Can I send samples that have already undergone mitochondrial isolation?

Yes. We accept both raw tissues/cells and pre-isolated mitochondrial fractions, provided you supply basic QC documentation (e.g., Western blot or protein concentration).

How do you ensure mitochondrial purity for accurate lipid profiling?

We use Western blot detection of mitochondrial markers (e.g., TOM20, COX IV) and cytosolic/membrane exclusion markers (e.g., GAPDH, calnexin) to confirm mitochondrial enrichment.

Can you analyze specific lipid classes only (e.g., cardiolipins)?

Absolutely. Upon request, we can tailor the analysis to focus on specific lipid categories or pathways and adjust quantification and method parameters accordingly.

How are the internal standards selected for normalization?

We use class-specific, isotopically labeled lipid standards to correct for extraction efficiency, ion suppression, and quantification accuracy across different lipid subclasses.

Is batch analysis possible for multiple sample groups or time points?

Yes. We support multi-group and longitudinal sample design, with consistent processing and batch-correction across all conditions.

What file formats will I receive for downstream analysis?

You'll receive processed Excel tables, raw instrument files (.raw or .wiff), statistical plots (.png/.pdf), and optional annotated pathway diagrams (.svg/.pdf).

Do you provide support for publication figures or formatting?

Yes. We offer graphical abstract design, figure formatting, and legend drafting tailored for journals in biology, metabolism, or lipidomics.

How stable are mitochondrial lipids during sample transport?

Lipid integrity is best preserved by snap-freezing samples in liquid nitrogen and shipping on dry ice. Avoid freeze-thaw cycles to prevent lipid degradation.

Can I combine mitochondrial lipidomics with proteomics or metabolomics?

Yes. We provide integrated mitochondrial omics platforms, allowing for coordinated lipid–protein–metabolite profiling from the same sample batch.

How long will it take to receive the results?

Turnaround time is typically 4–8 weeks, depending on sample number and analysis depth. Rush service is available upon request.

Publications

White matter lipid alterations during aging in the rhesus monkey brain. GeroScience, 2024. https://doi.org/10.1007/s11357-024-01353-3
Characterization of Dnajc12 knockout mice, a model of hypodopaminergia. bioRxiv, 2024. https://doi.org/10.1101/2024.07.06.602343
Annexin A2 modulates phospholipid membrane composition upstream of Arp2 to control angiogenic sprout initiation. The FASEB Journal, 2023. https://doi.org/10.1096/fj.202201088R
Lipid Membrane Engineering for Biotechnology (Doctoral dissertation, Aston University). Lipid Membrane Engineering for Biotechnology, 2023. https://doi.org/10.48780/publications.aston.ac.uk.00046663
Multi‐omics identify xanthine as a pro‐survival metabolite for nematodes with mitochondrial dysfunction. EMBO Journal, 2019. https://doi.org/10.15252/embj.201899558
Evidence for phosphate-dependent control of symbiont cell division in the model anemone Exaiptasia diaphana. mBio, 2024. https://doi.org/10.1128/mbio.01059-24
The olfactory receptor Olfr78 promotes differentiation of enterochromaffin cells in the mouse colon. EMBO reports, 2024. https://doi.org/10.1038/s44319-023-00013-5
Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty. Fabad Journal of Pharmaceutical Sciences, 2023. https://doi.org/10.55262/fabadeczacacilik.1344851
Prospective randomized, double-blind, placebo-controlled study of a standardized oral pomegranate extract on the gut microbiome and short-chain fatty acids. Foods, 2023. https://doi.org/10.3390/foods13010015