Lysophosphatidylcholine (LPC) is an intermediate product of lecithin metabolism in the body. LPC has strong surface activity and therefore can rupture red blood cells and other cell membranes, causing hemolysis or cell necrosis. Lysophosphatidylcholine acyltransferase catalyzes the acylation of LPC to form phosphatidylcholine (PC).
LPC plays a biological role by binding to G protein-coupled receptors and Toll-like receptors. LPC can induce the migration of lymphocytes and macrophages, increase the production of pro-inflammatory cytokines, induce oxidative stress and promote cell apoptosis, thereby intensifying inflammation and promoting the development of diseases. The effect of LPC on endothelial cells, vascular smooth muscle cells and arteries plays a vital role in the progression of atherosclerosis and other cardiovascular diseases. The regulation of inflammation by LPC plays different roles in inflammation and infectious diseases. Targeting LPC and lipid metabolism may be a potential treatment for inflammation-related diseases.
In addition, LPC content is an important quality control index for drugs, especially for injectables, and is directly related to the clinical safety of drugs. Therefore, its content needs to be strictly controlled.
Fig 1. Difference in plasma lipid profiles between the obese and normal-weight groups (Yang Wang et al.,2019)
Creative Proteomics uses the LC-MS/MS analysis platform to establish a stable, sensitive and reliable detection platform for quantitative detection of target LPC, which can help promote the progress of related projects.
Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.
High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for lysophosphatidylcholine analysis service (Triple Quad™ 6500 LC-MS/MS System).
Fig 2. The workflow of lysophosphatidylcholine analysis service
We routinely cover a rich variety of sample types, including cells, cell medium, tissue, blood, plasma for our customer. If you provide other samples, please contact our technicians for specific quantities and treatment methods.
If you have any questions about our lysophosphatidylcholine detection service, please contact us.