Very long chain fatty acid (VLCFA) is a fatty acid with a carbon number of more than 20. Ultra-long-chain fatty acids are precursors of lipid media, and are also constituents of cellular lipids such as sphingolipids and glycerophospholipids. Mutations in genes encoding extra-long-chain fatty acid metabolism enzymes can lead to a series of genetic diseases, such as ichthyosis, demyelination, myopathy, mental retardation, and macular degeneration. The identification of enzymes related to the synthesis and degradation of ultra-long-chain fatty acids can increase our understanding of the functions of ultra-long-chain fatty acids.

Ultra-long-chain fatty acids can be produced in all plant cells. Ultra-long-chain fatty acids are produced in specific cell types and are precursors or components of a large number of metabolites.

Creative Proteomics provides comprehensive very long chain fatty acids targeted lipidomics services from lipid extraction, lipid detection and data analysis for biomedical research institutions, biotechnology and pharmaceutical companies.

Fig 1. Scheme representation of very-long-chain fatty acid elongation (De Bigault, 2016)

Our Very Long Chain Fatty Acids Analysis Service

Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.

High sensitivity: we use liquid chromatography coupled with triple quadrupole mass spectrometers for very long chain fatty acids analysis service (Triple Quad™ 6500 LC-MS/MS System).

Fig 2. The workflow of very long chain fatty acids targeted lipidomics.

Detectable Saturated Fatty Acids Include But Are Not Limited To

Sample Requirements

• Serum/plasma ≥ 50 µL
• Animal and plant tissues ≥ 50 mg
• Microorganism ≥ 0.2 g/sample
• Fermentation broth ≥ 20 ml/sample
• Fresh stool ≥ 50 mg
• Freeze-dried stool ≥ 5 mg
• Cell ≥ 1×10⁷
• Food samples need to be in more than 10 groups

If you provide other samples or want to know the specific sample size, please contact us.

Why Choose Us?

• One-stop analysis service: efficient lipid molecule detection. Save time, energy and money.
• Fast turnaround time: 1-4 weeks.
• Detailed delivery report: Experiment procedures and parameters of instruments, MS data with putative identification based on the m/z ratio of the analytes, and customized bioinformatics analysis.

If you have any questions about our lipidomics service, please contact us.

Reference:

1. De Bigault Du Granrut A.; Cacas J L. How very-long-chain fatty acids could signal stressful conditions in plants? Frontiers in plant science. 2016, 7: 1490.

Resource:

Platform:

• Mass Spectrometry Systems
• Chromatographic Systems
• Shot-Gun Lipidomics
• GC/LC-MS Lipidomics
• Sample Guidelines