Sophorolipids Analysis Service
Creative Proteomics has developed a reliable and reproducible method using highly sensitive LC-MS/MS method for the rapid identification and quantification of Sophorolipids (SLs) in yeast, which can satisfy the needs of academic and industrial study in your lab.
Overview
Biosurfactants are one promising substance class currently under investigation. Sophorolipids (SLs) are biosurfactants of microbial origin, which show biodegradability, low toxicity, excellent surface-active properties, and biological activities. Sophorolipids, secondary metabolites mainly secreted by non-pathogenic yeasts, are one of the most promising biosurfactants. Structurally, they are composed of a disaccharide sophorose linked by a β-glycosidic bond to a long fatty acid chain. In fermentation broth, SLs are synthesized as a mixture of slightly different molecules with three major points of variation of the lactonization, acetylation pattern, and the fatty acid part (chain length, saturation, and position of hydroxylation). The different structural classes cause wide variation in physicochemical and biological properties. Nowadays, SLs have been reported to apply in fields such as agriculture, food, biomedicine, bioremediation, cosmetics, nanotechnology, and oil.
The combination of MS instrument and liquid chromatography (LC) can effectively separate target lipid molecules from complex samples. Currently, a reliable and reproducible method using highly sensitive LC-MS/MS platform for the identification and quantification of SLs in yeast has been established by the scientists at Creative Proteomics, which can satisfy the needs of academic and industrial study in your lab.
Why Choose Us?
- With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective SLs targeted lipidomics services.
- Send us your sample and receive data in 2-4 weeks.
- Our project is a very complex study that requires a high degree of sensitivity and accuracy. Creative Proteomics has molecular biologists, mass spectrometry specialists, bioinformatics experts, and lipidomics data scientists. We are well-prepared and positioned to foster collaboration in the fast-developing field of lipidomics.
Our Sophorolipids Analysis Service
Fig 2. The workflow of sophorolipids analysis service
Structural details: species level
Mode: MRM
Precision: pmol & mol%
Delivery time: 2-4 weeks
Deliverables
- Experimental procedure
- Parameters of liquid chromatography and MS
- Purity analysis report
- MS raw data files and MS data quality checks
- Hexylceramide quantitative result data
- Custom analysis report
Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, Creative Proteomics provides reliable, fast and cost-effective sophorolipids analysis services. If you have any questions about our sophorolipids targeted lipidomics services, please contact us.
Reference:
- Ma, Xiaojing.; et al. Sophorolipid biosynthesis and production from diverse hydrophilic and hydrophobic carbon substrates. Applied microbiology and biotechnology. 2020, 104.1: 77-100.
* Our services can only be used for research purposes and Not for clinical use.
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