Conditioned medium is spent media harvested from cultured cells. It contains metabolites, growth factors, and extracellular matrix proteins secreted into the medium by the cultured cells. Lipids are an important part of conditioned medium. Different cells secrete different lipids into the medium during the culture process, and these lipids can affect other cells in a paracrine or autocrine manner.
Studies have shown that compared with normal fibroblast conditioned medium, colorectal cancer tumor-associated fibroblast conditioned medium contains more fatty acids and phospholipids, which promotes the metastasis of colorectal cancer cells.
Fig 1. The mechanism of cancer-associated fibroblasts in tumor metastasis (Gong et al., 2020)
Therefore, identification the lipid profiles of conditioned medium of different cells or different states of the same cell can offer novel biomarkers for specific disease, also provide new insights for us to deeply understand the pathogenic mechanism of diseases and drug resistance mechanism.
Creative Proteomics provides comprehensive services from conditioned medium collection, lipid extraction, lipid detection and data analysis for biomedical research institutions, biotechnology and pharmaceutical companies.
Creative Proteomics performs untargeted lipidomic analysis of conditioned medium samples using liquid chromatography-mass spectrometry (LC-MS/MS). Univariate analysis of variance is used to examine differences in lipid categories and individual lipids between each group of samples.
Fig 2. The workflow of conditioned medium lipidomics analysis service.
We need 200-600 ul of conditioned medium for our untargeted lipidology services. The specific sample requirements are ultimately determined based on the experimental design.