Untargeted Lipidomics Service

Lipidomics can study the structure, function, and interaction of various lipids, as well as the regulation of the physiological and pathological states of the body. Untargeted lipidomics helps to discover important key lipid metabolites in the body, clarify the relationship between lipids and various diseases, and promote the study of the mechanism of various life activities.

Creative Proteomics provides untargeted lipidomics services, which can separate and identify all lipid compounds and their metabolites in biological samples to screen for biomarkers. We provide untargeted lipidomics services for many fields, including agricultural science, biomarkers, Alzheimer's disease, cancer, diabetes and obesity research, clinical diagnosis, drug discovery, and systems biology.

Our Untargeted Lipidomics Services

  • Shot-gun untargeted lipidomics

Shot-gun lipidomics directly introduces lipids and detects the lipids by MS, with separation of lipids achieved solely on the basis of mass-to-charge ratio. We use a new shotgun lipidomics platform was established by combining an Orbitrap Fusion mass spectrometer with a TriVersa Nanomate nanoelectrospray ion source. One example of the shotgun workflow is demonstrated below.

The workflow of shot-gun untargeted lipidomics.Fig1. The workflow of shot-gun untargeted lipidomics. (Creative Proteomics)

Advantages of shot-gun strategy

  • No LC separation, fast, minimum sample consumption.
  • Up to 500,000 resolution removes interferences, 1ppm mass accuracy.
  • A four-pole mass filter is used to allow the ion trap and orbitrap mass analyzer to operate in parallel, thereby obtaining excellent sensitivity and selectivity
  • Multiple dissociation techniques—CID, HCD, and optional ETD—with ion trap or Orbitrap detection at any level of MSn maximize flexibility for research applications.
  • LC-MS Untargeted Lipidomics

Although shot-gun lipidomics can answer many of the needs associated with sample lipid profiling, some workflows do require chromatographic separation before MS analysis. For example, the quantification of isobaric lipids can only be done if the lipids are somehow separated before entering the mass spectrometer.

In these cases, we would choose a column that is suitable for the separation of your lipids of interest and use ultrahigh-performance liquid chromatography equipped with different mass spectrometry systems to analyze the untargeted lipidomics. One example of the LC-MS lipidomics workflow is demonstrated below.

The protocol workflow of LC-MS untargeted lipidomics.Fig2. The protocol workflow of LC-MS untargeted lipidomics. (Creative Proteomics)

Advantages of LC-MS strategy

  • The ACQUITY UPLC System can reduce ion suppression caused by coeluted lipids and isobaric interferences, and it can often separate isomers.
  • Chromatographic separation before an MS analysis is still necessary for analyzing very low-abundance signaling lipids.
  • A partial chromatographic separation helps avoid the isotopic effects, which affect the actual mass abundance during direct-infusion experiments.

Featured Untargeted Lipidomics Analysis Service

Sample Requirements

1. Serum/plasma/urine/ruminal fluid/cerebrospinal fluid/amniotic fluid and other homogeneous liquid samples: ≥ 200ul/sample
2. Animal tissue/feces related samples: ≥ 200mg/sample
3. Plant roots/stems/leaves/fruits/seeds: ≥ 200mg/sample
4. Cells/microorganisms: ≥ 10^7/sample
5. Cell supernatant/culture medium supernatant ≥ 2mL or more/sample
Store at -80℃ after quick freezing in liquid nitrogen; send on dry ice.

Why Choose Us?

  • Creative Proteomics designs experimental programs according to customer needs and choose different technical methods to improve detection efficiency and ensure the accuracy and repeatability of experimental results.
  • A wide range of samples can be analyzed, including subcellular compartments, bacteria, yeast and other microorganisms, cultured cells, skin patches, body fluids, blood and plasma, tissues and organs: liver, brain, etc., as well as oil and milk, plants, food, etc.
  • Provide a detailed report at the end of the entire project, including experimental procedures, MS/MS instrument parameters, raw data, and bioinformatics analysis to help you find differential lipid molecules.

If you have any questions about our untargeted lipidomics services, please contact us.

* Our services can only be used for research purposes and Not for clinical use.

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