Lipidomics can study the structure, function, and interaction of various lipids, as well as the regulation of the physiological and pathological states of the body. Untargeted lipidomics helps to discover important key lipid metabolites in the body, clarify the relationship between lipids and various diseases, and promote the study of the mechanism of various life activities.
Creative Proteomics provides untargeted lipidomics services, which can separate and identify all lipid compounds and their metabolites in biological samples to screen for biomarkers. We provide untargeted lipidomics services for many fields, including agricultural science, biomarkers, Alzheimer's disease, cancer, diabetes and obesity research, clinical diagnosis, drug discovery, and systems biology.
Shot-gun lipidomics directly introduces lipids and detects the lipids by MS, with separation of lipids achieved solely on the basis of mass-to-charge ratio. We use a new shotgun lipidomics platform was established by combining an Orbitrap Fusion mass spectrometer with a TriVersa Nanomate nanoelectrospray ion source. One example of the shotgun workflow is demonstrated below.
Fig1. The workflow of shot-gun untargeted lipidomics. (Creative Proteomics)
Although shot-gun lipidomics can answer many of the needs associated with sample lipid profiling, some workflows do require chromatographic separation before MS analysis. For example, the quantification of isobaric lipids can only be done if the lipids are somehow separated before entering the mass spectrometer.
In these cases, we would choose a column that is suitable for the separation of your lipids of interest and use ultrahigh-performance liquid chromatography equipped with different mass spectrometry systems to analyze the untargeted lipidomics. One example of the LC-MS lipidomics workflow is demonstrated below.
Fig2. The protocol workflow of LC-MS untargeted lipidomics. (Creative Proteomics)
1. Serum/plasma/urine/ruminal fluid/cerebrospinal fluid/amniotic fluid and other homogeneous liquid samples: ≥ 200ul/sample
2. Animal tissue/feces related samples: ≥ 200mg/sample
3. Plant roots/stems/leaves/fruits/seeds: ≥ 200mg/sample
4. Cells/microorganisms: ≥ 10^7/sample
5. Cell supernatant/culture medium supernatant ≥ 2mL or more/sample
Store at -80℃ after quick freezing in liquid nitrogen; send on dry ice.
If you have any questions about our untargeted lipidomics services, please contact us.