Fatty Acid Methyl Ester Analysis

Fatty acid and fatty acid methyl ester analysis can be applied to various industries.

In the field of food nutrition and food chemistry, GC and LC analysis of fatty acids and their methyl ester derivatives (FAME) are important tools for fat characterization.

In the chemical industry, fatty acid methyl esters are important chemical raw materials. FAME obtained from the methyl esterification of vegetable oils or other oils are popular for biodiesel fuels. There are differences in composition and content of biodiesel made from raw materials of different sources. An accurate understanding of the composition and content of mixed fatty acid methyl esters is helpful for the quality control of biodiesel and subsequent products.

The fatty acid components commonly contained in microbial cell structure have a high degree of homology with microbial DNA, and various microbes have their characteristic cellular fatty acid fingerprints. Analyzing the phospholipid fatty acid on the cell membrane of microorganisms can promote the detection and identification of microbial diversity and the study of microbial diversity.

Creative Proteomics has established a fatty acid methyl ester analysis platform, using LC-MS/MS and GC-MS/MS technologies to achieve efficient and accurate composition and quantitative analysis of fatty acid methyl esters.

List of detectable fatty acid methyl ester at Creative Proteomics

Methyl butyrate (C4:0)Methyl 9-cis-tetradecenoate(C14:1n5)Methyl oleate (C18:1n9c)Methyl cis-11,14-eicosadienoate (C20:2n6)Methyl cis-13,16-docosadienoate (C22:2n6)
Methyl caproate (C6:0)Methyl pentadecanoate (C15:0)Methyl oleate (C18:1n9t)Methyl cis 8,11,14-eicosatrienoate (C20:3n6)DHA methyl ester (C22:6n3)
Methyl caprylate (C8:0)Methyl cis-10-pentadecenoate (C15:1n5)Methyl linoleate (C18: 2n6c)Methyl cis-11,14,17-eicosatrienoate (C20:3n3)Methyl tridecanoate (C23:0)
Methyl decanoate (C10:0)Methyl palmitate (C16:0)Trans-linoleic acid methyl ester (C18: 2n6t)Methyl arachidonic acid (C20:4n6)Methyl lignocerate (methyl tetracosanoate) (C24:0)
Methyl undecanoate (C11:0)Methyl palmitoleate (C16:1n7)Methyl γ-linolenate (C18:3n6)Cis-5,8,11,14,17-eicosapentaenoic acid methyl ester (C20:5n3)Methyl tetradecenoate (C24:1n9)
Methyl laurate (C12:0)Methyl heptadecanoate (C17:0)Methyl α-linolenate (C18:3n3)Methyl Eicosanate (C21:0) 
Methyl tridecanoate (C13:0)Methyl cis-10-heptadecenoate (C17:1n7)Methyl arachidate (methyl eicosanate) (C20:0)Methyl behenate (C22:0) 
Methyl myristate (C14:0)Methyl stearate (C18:0)Methyl cis-11-eicosenoate (C20:1)Methyl parasinate (C22:1n9) 

Technology platform

  • High performance liquid chromatography (HPLC)
  • Gas chromatography-mass spectrometry (GC-MS)
  • Liquid chromatography-mass spectrometry (LC-MS)

A typical workflow of mass spectrometry-based lipidomicsA typical workflow of mass spectrometry-based lipidomics (Wang et al., 2019)

Sample requirements

  • Blood/plasma: ≥ 500 ul/sample
  • Urine: ≥ 1 ml/sample
  • Animal tissue: ≥ 200 mg/sample
  • Plant tissue: ≥ 200 mg/sample
  • Microorganism ≥ 200 mg/sample

If you want to analyze other samples, please contact our experts for consultation.


  • Experimental procedure
  • Parameters of gas chromatography, liquid chromatography, mass spectrometry
  • MS raw data files and MS data quality checks
  • Spectrum of fatty acid methyl ester analysis
  • Custom analysis report

Delivery time: 2-4 weeks

Creative Proteomics has many years of experience in lipidomics analysis services, and enjoys a good reputation in supporting the detection and quantification of various lipids. Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, we provide reliable, fast and cost-effective fatty acid methyl ester analysis services. If you have other substances you want to test or other questions, please contact us.

* Our services can only be used for research purposes and Not for clinical use.




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