Lipidomics in MAFLD, NASH & Cardiovascular Research

Emerging Trends

Recent studies, such as the work by Felix, J. B., et al. (2025) in Nature Metabolism, have highlighted that adipocyte-derived metabolites like N-acetylaspartate act as critical signaling molecules that regulate systemic energy balance and postprandial thermogenesis. This discovery emphasizes that Lipidomics in MAFLD/NASH research must go beyond simple triacylglycerol (TAG) profiling to capture the subtle shifts in regulatory lipid species that orchestrate organ-to-organ communication during the progression from simple steatosis to non-alcoholic steatohepatitis (NASH).

Furthermore, the pathogenesis of atherosclerosis is increasingly recognized as a lipid-driven inflammatory process. The dysregulation of cholesterol trafficking and the accumulation of oxidized phospholipids within the arterial wall are primary triggers for plaque instability. By employing Untargeted Lipidomics, researchers can identify unique lipid signatures that differentiate stable from vulnerable plaques. This global approach allows for the discovery of lipid mediators involved in the lipid homeostasis mechanism, which can serve as early warning signs of cardiovascular events.

Quantitative Accuracy and Sensitivity

By utilizing Sciex QTRAP 6500+ and Orbitrap Exploris 480 systems, we achieve lower limits of quantification (LLOQ) in the fmol/mL range. This sensitivity is critical for detecting low-abundance signaling lipids in cardiovascular tissues. We consistently achieve commonly reported coefficients of variation (CV) below 15% across large-scale cohorts.

Isomeric Resolution via TIMS

Our Trapped Ion Mobility Spectrometry (TIMS) coupled with high-resolution LC provides an extra dimension of separation. This allows for the differentiation of lipids with identical mass-to-charge ratios but distinct collisional cross-sections (CCS), which is indispensable for the accurate lipid homeostasis mechanism assessment.

Sample Integrity and Antioxidant Protocols

Recognizing the fragility of oxidized lipids in NASH and atherosclerosis research, we implement a strict cold-chain extraction process. Samples are processed at 4°C with the immediate addition of BHT and EDTA to prevent the artificial formation of lipid peroxides.