Creative Proteomics has developed a reliable and reproducible method using highly sensitive LC-MS/MS method for the rapid identification and quantification of CerEOP in skin sample, which can satisfy the needs of academic and industrial study in your lab.
Overview
Omega-hydroxy fatty acids [EO] Phytosphingosine [P] Ceramides (CerEOP or EOP) are a subclass of acylceramides, which are composed of phytosphingosine and fatty acids. The role of CerEOP is mainly related to human skin, and they mainly achieve structural purposes. CerEOP is related to the formation of the lamellar phase of the epidermal barrier to prevent transdermal water loss, while also protecting the environment. Distortion of skin ceramide distribution can cause or contribute to skin conditions. Generally speaking, decreased omega hydroxyceramide levels are associated with dry skin and psoriasis.
Tape stripping is an almost non-invasive method with the benefits of controlling the sampling depth and collecting comparable sample amounts. This enables collecting a high number of samples and allows screening and biomarker studies, increasing much needed statistical validity of results and conclusions. CerEOP samples, collected by tape stripping of human skin, were analyzed by highly sensitive LC-MS/MS method in Creative Proteomics.
Advantages of our CerEOP analysis service
- With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective CerEOP targeted lipidomics services.
- Send us your sample and receive data in 3-6 weeks.
- Our project is a very complex study that requires a high degree of sensitivity and accuracy. Creative Proteomics has molecular biologists, mass spectrometry specialists, bioinformatics experts, and lipidomics data scientists. We are well-prepared and positioned to foster collaboration in the fast-developing field of lipidomics.
- Creative Proteomics full-service facility provides data processing (e.g., data alignment, feature detection), feature prioritization (e.g., principal component analysis, heat maps, ANOVA/t-test, multivariate statistical analysis, self organizing maps, volcano plots, and network activity prediction), and lipid identification services.
List of detectable CerEOP at Creative Proteomics
Our Process
Fig1. The protocol workflow of CerEOP targeted lipidomics (Creative Proteomics)
Structural details: species level
Precision: pmol & mol%
Variants identified: >10
Delivery time: 3-6 weeks
Deliverables
- Experimental procedure
- Parameters of liquid chromatography and MS
- Purity analysis report
- MS raw data files and MS data quality checks
- Hexylceramide quantitative result data
- Custom analysis report
Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, we provide reliable, fast and cost-effective CerEOP analysis services. If you have any questions about our CerEOP targeted lipidomics services, please contact us.
* Our services can only be used for research purposes and Not for clinical use.
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