Biological fluids such as saliva, cerebrospinal fluid, tears, aqueous humor, and amniotic fluid all contain lipids, which can provide information on lipid changes related to diseases.
|Saliva||The ease in availability, sampling and especially the relevance in pursuing as a source of biomarkers in oral cancer, diabetes and chronic oral inflammatory conditions such as periodontitis makes saliva an important biofluid to study disease associated changes in lipid profile.|
|Cerebrospinal Fuid||Cerebrospinal fluid (CSF) is considered a promising source of biomarkers for neurodegenerative diseases such as Alzheimer's, dementia, and chronic neurological diseases. It is anatomically close to the brain. CSF lipidomics analysis is suitable for exploring lipid homeostasis in brain diseases, thereby reflecting disease-related changes.|
|Tear||Tear fluid acts as a signboard either to understand the etiology of the ocular disease and/or to track the prognosis/response to treatment. Lipids, besides proteins are one of the major components of the tear film with an inner mucin layer, middle aqueous layer and the outer lipid layer. Our comprehensive lipidome analysis of tear covers 17 major lipid categories, including more than 600 lipid types.|
|Aqueous Humor||While tears immediately reflect the changes occurring at the cornea eyelids, aqueous humor alterations in tear constituents also reflects the disease mediated changes happening in the posterior portion of the eye.|
|Amniotic||Amniotic fluid lipid group can help understand the lipid changes in diseases such as spontaneous preterm labor and term delivery, term clinical chorioamnionitis, fetal hyperlipidemia, and respiratory distress syndrome, and accelerate the study of related disease mechanisms.|
Therefore, comprehensive lipidomic data from biofluids is likely to be point-of-care diagnostics for certain targets validated to be useful biomarkers in disease. Creative Proteomics provides comprehensive biofuild lipidomics services from lipid extraction, lipid detection and data analysis for biomedical research institutions, biotechnology and pharmaceutical companies.
Based on liquid chromatography-mass spectrometry (LC-MS) technology, creative proteomics unbiased detection of lipid molecules in biofluids samples.
Fig1. The workflow of biofuild untargeted lipidomics analysis service.
According to different experimental schemes, we usually need 1-200 ul biological samples for our untargeted lipidomics services.
Avoid repetitive freeze-thaw cycles, store at -80°C.