Hydroxycholesterols are mono-oxygenated derivatives of cholesterol. In vivo, hydroxycholesterols are present as unesterified (biologically active) and esterified forms (largely biologically inert).
Hydroxycholesterols are important intermediates in a number of catabolic pathways that regulate a variety of biological effects.
Hydroxycholesterols are critical for cholesterol transport from the periphery to the liver, regulate the expression of sterol sensitive genes which are involved in lipid and sterol biosynthesis, act as substrates for the formation of bile salts, serve as ligands that activate nuclear liver X receptors-α and -β, and are involved in the regulation of cholesterol and lipid metabolism and homeostasis.
Fig 1. Structural representation of hydroxycholesterols (Bandaru et al., 2014)
In the central nervous system, hydroxycholesterols regulate arachidonic acid release, voltage-gated calcium channels, synaptic plasticity, induce IL-8, promote neurogenesis and induce apoptosis.
Creative Proteomics provides comprehensive hydroxycholesterols analysis services from lipid extraction, lipid detection and data analysis for biomedical research institutions, biotechnology and pharmaceutical companies.
Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.
High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for hydroxycholesterols analysis service (Triple Quad™ 6500 LC-MS/MS System).
Fig 2. The workflow of hydroxycholesterols analysis service
|Normal volume||200 μL||100 mg||2E7||80mg- human|
|Minimum volume||100 μL||50 mg||5E6||50mg-human|
If you have any questions about our hydroxycholesterols analysis services, please contact us.