Phosphatidylserine (PS) is an important membrane phospholipid found in bacterial, yeast, plant, and mammalian cells. It is usually located in the inner layer of the cell membrane and is associated with a range of membrane functions. Among the many phospholipid components, PS is the only phospholipid that can regulate the functional state of key proteins of the cell membrane. Especially in the human nervous system, it is one of the important components of the cell membrane of the brain and plays an important role in regulating various functions of the brain (especially memory and emotional stability).
As one of the main components of the brain nerves, PS can nourish and activate the activity of various enzymes in the brain, slow down the process of neurotransmitter reduction, help repair and renew damaged brain cells and remove harmful substances. It can positively affect the treatment of pediatric ADHD caused by brain tonic damage, malnutrition and hereditary decrease in the number of neurotransmitters.
Alzheimer's disease has become a worldwide problem that cannot be ignored. As we age, phosphatidylserine and other important chemicals that affect brain function gradually decrease, and this chemical change leads to a decrease in enzyme activity and transport capacity in normal cell membranes, as well as a decrease in nerve nodes, thus leading to a decline in memory and cognitive ability. Supplementation of PS can increase the number of synapses, enhance the mobility of brain cell membranes, and promote glucose metabolism in brain cells, resulting in more active brain cells.
Tension depression activity is also transmitted through neurotransmitters. According to neurotransmitter theory, norepinephrine system and 5-hydroxytryptaminergic system dysfunction in the brain may be the cause of tension and depression. Phosphatidylserine significantly reduces excessive levels of stress hormones in people who are stressed at work, reduces stress and relieves brain fatigue, and also promotes concentration, alertness and memory, and relieves bad moods.
Many key signal transduction proteins require PS as enzyme cofactors to achieve optimal activities, such as the activation of protein kinase C (PKC). The pre-activated PKC primase is located in the cytoplasm and is in a blunted state. It is transferred from the cytoplasm to the cell membrane by the action of diphosphoglycerol (DAG) and becomes in a state to be activated. The enzyme catalyzes the formation of activated PKC-PS-DAG-Ca2+ complexes with Ca2+, PS and DAG. Activated proteinase kinase C phosphorylates serine and threonine residues, thereby affecting biological information transmission.
Whether it is the quantitative analysis of PS in animal and plant cells, body fluids, bacteria, yeast, or the determination of PS in food or nutraceuticals, the LC-MS/MS technology platform can be used for efficient and accurate qualitative and quantitative analysis. The advantages of LC-MS/MS technology are the LC's ability to separate a wide range of compounds and the MS's ability to quantify compounds with high sensitivity and selectivity based on unique mass/charge (m/z) transitions.
Based on the LC-MS/MS technology platform, Creative Proteomics provides qualitative and quantitative phosphatidylserine analysis services to customers in the pharmaceutical, food, and bioscience industries.