Ether-linked phosphatidylethanolamine (PE O-) are a type of glycerophosphoethanolamines, a class of glycerophospholipids. Their structure consists of a glycerol backbone linked to two fatty acids and a phosphoethanolamine molecule. One of the fatty acids is bound to the glycerol backbone via an ether bond. The fatty acids can be of variable length, hydroxylated, and contain double bonds.
Ether-linked phosphatidyl-ethanolamines contain high amounts of arachidonic acid required for the biosynthesis of eicosanoids, bioactive lipids with a great role in inflammation. Levels of PE O- lipids are reduced in the blood of patients with multiple sclerosis. That said, elevated levels have been found in cancers, especially in aggressive forms. Further, PE O- lipids are important membrane constituents of neutrophils, the most abundant type of white blood cells in humans.
With extensive experience and state-of-the-art technologies, Creative Proteomics use highly sensitive LC-MS/MS method for the rapid identification and quantification of ether-linked phosphatidylethanolamine in different sample types, which can satisfy the needs of academic and industrial study.
Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.
High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for ether-linked phosphatidylethanolamine analysis service (Triple Quad™ 6500 LC-MS/MS System).
Fig 2. The workflow of ether-linked phosphatidylethanolamine analysis service
Please inform us of any preservatives or additives used in the collection process. Use Sharpie to directly label tubes because the label may fall off during freezing.
Creative Proteomics has molecular biologists, mass spectrometry specialists, bioinformatics experts, and lipidomics data scientists. We are well-prepared and positioned to foster collaboration in the fast-developing field of lipidomics. If you have any questions about our ether-linked phosphatidylethanolamine detection service, please contact us.