Eicosanoid Analysis Service

Creative Proteomics has developed a reliable and reproducible method using highly sensitive LC-MS/MS method for the rapid identification and quantification of eicosanoid in different sample types, which can satisfy the needs of academic and industrial study in your lab.

Overview

Eicosanoids are a large group of biologically active unsaturated fatty acids produced by the oxidation of eicosanoid polyunsaturated fatty acids, including prostaglandin (PG), thromboxane (TX), leukotriene (LT) and lipoxins (LX). They are all derived from arachidonic acid (AA), which contains twenty carbons and has carboxyl groups.

Eicosanoid is an important inflammatory factor, which is widely present in body fluids and tissues, and regulates many physiological and pathological processes in the body. Eicosanoids play an important role in the inflammation of joints, skin and eyes, the induction of childbirth, and the intensity and duration of pain and fever. They also help to inhibit gastric acid secretion, regulate blood pressure through vasodilation or contraction, and inhibit or activate platelet aggregation and thrombosis. Eicosanoids can be synthesized in all mammalian cells except red blood cells. A small amount of eicosanoid can exert a powerful physiological effect. At the synthesis site, eicosanoids exert their local effects through receptor-mediated G protein connection signaling pathways.

Eicosanoids play a vital role in many diseases, so it is very meaningful to detect and quantify these compounds. Such compounds are produced and released quickly under various physiological and pathological stimuli, and have a small synthesis amount, poor stability, and a large number of isomers. Therefore, the separation and analysis of eicosanoids in organisms poses a greater challenge. Sensitive and specific analytical methods are needed to quantify these compounds. HPLC-MS/MS has become one of the main techniques for the quantification of eicosanoids.

Creative Proteomics is based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS to provide reliable, fast and cost-effective analysis services for eicosanoids.

Advantages of Our Eicosanoid Analysis Service

  • One-stop analysis service. From sample preparation, mass spectrometry, data processing to the identification and quantification of eicosanoids.
  • Rich experience in sample processing. A variety of sample types can be accepted, including tissues, blood, cells, body fluids, etc.
  • Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.
  • Flexible statistical analysis and bioinformatics analysis.

Our Eicosanoid Analysis Service

workflow of eicosanoid analysis serviceFig 1. The workflow of eicosanoid analysis service

Structural details: species level
Precision: pmol & mol%
Delivery time: 1-4 weeks

Sample Requirements

Blood/plasma: 500 ul/sample
Tissue: 200 mg/sample
Cells: 1x107/sample

  • A minimum of 6 replicate plates or wells per condition are required.
  • After the sample is collected, it will be stored at -80°C immediately and transported by dry ice.

Deliverables

  • Experimental procedure
  • Parameters of liquid chromatography and MS
  • Purity analysis report
  • MS raw data files and MS data quality checks
  • Eicosanoid quantitative result data
  • Custom analysis report

Due to its low abundance and structural variability, the quantification of endogenous dodecanoic acids is challenging. With many years of experience in lipidomics analysis services, Creative Proteomics has a reputation for supporting the detection and quantification of multiple lipids. If you have any questions about our eicosanoid analysis service, please contact us.

* Our services can only be used for research purposes and Not for clinical use.

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