Platelets are composed of a variety of lipids, including phospholipids, sterols, sphingolipids, free fatty acyl, and glycerol.
Phospholipids are the major lipid classes in platelets and include phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidylglycerol (PG), and phosphatidic acid (PA).
Sterol lipids within platelets include cholesterol and cholesteryl esters (CEs). Altered platelet cholesterol content leads to proportional changes in agonist-induced aggregation. Cholesterol is a necessary component of lipid rafts.
The main sphingolipids present in platelets are ceramide and sphingomyelin. Sphingolipids regulate the ability of intracellular calcium (Ca2+) storage. In addition, sphingolipids are also rich in lipid rafts that provide a platform for mediating signal transduction, particularly ceramides.
Free fatty acyl present in platelets include arachidonic, linoleic, eicosapentaenoic, docosapentaenoic, docosahexaenoic, dihydro-γ-linolenic, palmitic, stearic, and oleic acids. Triacylglycerides (TAGs) and diacylglycerides (DAGs), although representing only a small fraction of platelet liposomes, represent an important and potent group of bioactive lipid mediators.
Platelets have functions such as initiating wound healing, in addition to playing an important role in cancer development, immune regulation and activation of thrombin-dependent aggregation. Some of the functions of platelets are closely related to the lipid composition and changes in platelets. The interactions between platelets, extracellular vesicles and storage fluid together contribute to the dynamic state of the lipid profile of the entire platelet composition. Analysis of the lipid profile of platelets is important for blood storage and blood transfusion.
Creative Proteomics provides platelet lipidomics solutions to explore information on lipid profiles and lipid isomers in platelets and to facilitate scientific research progress.
Creative Proteomics has built several platforms for lipidomic assays, including GC-FID, GC-MS and LC-MS, depending on the purpose of the assay and the type of analyte. Data analysis includes PCA, PLS-DA, PLS-R, and univariate statistics.
Fig1. The workflow of platelet lipidomics service.
Serum/plasma ≥ 200 ul /sample
Store at -80℃ after quick freezing in liquid nitrogen; send on dry ice.
If you have any questions about our platelet lipidomics services, please contact us.