Ergosteryl Esters Analysis Service
With extensive experience and state-of-the-art technologies, Creative Proteomics use highly sensitive LC-ESI/MS method for the rapid identification and quantification of ergosteryl esters in fungi, which can satisfy the needs of academic and industrial study.
Overview
Ergosterol ester (EE) is a sterol lipid. Their structure consists of ergosterol, in which an ester bond is formed between the hydroxyl group of the steroidal structure and the fatty acid. Fatty acids can be variable length, hydroxylated and contain double bonds.
The biological function of ergosterol esters mainly revolves around the storage of ergosterol in yeast. Under conditions of growth retardation and after the death of the fungus, ergosterol is esterified and stored in cellular lipid droplets. Once esterified, the fatty acids of ergosterol esters will not be removed even under starvation conditions. In addition, some ergosterol esters also show strong growth inhibitory activity against deteriorating bacteria in dairy products and cosmetics.
The combination of MS instrument and liquid chromatography (LC) can effectively separate target lipid molecules from complex samples. Currently, a reliable and reproducible method using highly sensitive LC-MS/MS platform for the identification and quantification of EE in fungi has been established by the scientists at Creative Proteomics, which can satisfy the needs of academic and industrial study in your lab.
Advantages of Our Ergosterol Ester Analysis Service
- One-stop services, including lipid extraction, lipid detection and data analysis. Cutting-edge apparatuses, reliable data and reproducible process.
- Our project is a very complex study that requires a high degree of sensitivity and accuracy. Creative Proteomics has molecular biologists, mass spectrometry specialists, bioinformatics experts, and lipidomics data scientists. We are well-prepared and positioned to foster collaboration in the fast-developing field of lipidomics.
List of Detectable Ergosterol Ester at Creative Proteomics
The Technical Process of Our Ergosterol Ester Analysis Service
The workflow of ergosterol ester analysis service
Structural details: species level
Precision: pmol & mol%
Variants identified: >25
Delivery time: 2-4 weeks
Sample requirements
- 200–350 mg dry weight/sample
- A minimum of 6 replicate plates or wells per condition are required.
- After the sample is collected, it will be stored at -80°C immediately and transported by dry ice.
- Tubes should be clearly labeled with sample identifiers and include a hard copy of the list of samples to accompany the shipment. Please include all available sample information when shipping samples.
Deliverables
- Experimental procedure
- Parameters of liquid chromatography and MS
- Purity analysis report
- MS raw data files and MS data quality checks
- Hexylceramide quantitative result data
- Custom analysis report
With years of experience in lipidomics analysis services, Creative Proteomics has a good reputation in supporting the detection and quantification of a variety of lipids. Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, we provide reliable, fast and cost-effective EE analysis services. If you have any questions about our EE targeted lipidomics services, please contact us.
* Our services can only be used for research purposes and Not for clinical use.
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