Lipoproteins Analysis Service
Lipoproteins are a class of spherical particles consisting of a hydrophobic core rich in sterolipids and triglycerides and a shell composed of proteins, phospholipids, and cholesterol. Lipoproteins play an important role in the packaging, storage, transport and metabolism of extracellular lipids in insects and mammals. Depending on their density, size and protein composition, lipoproteins can be classified into five main categories: celiac particles (CM), very low density lipoproteins (VLDL), intermediate density lipoproteins (IDL), low density lipoproteins (LDL) (mainly apoB-containing lipoproteins), and high density lipoproteins (HDL) (mainly apoA1-containing lipoproteins).
Abnormalities in lipoprotein metabolism (usually accompanied by alterations in the lipid and protein fractions) are strongly associated with atherosclerosis, diabetes, obesity, and tumorigenesis. The lipid fractions of lipoproteins are mainly cholesterol and triglycerides from dietary intake and hepatic synthesis. Of these, cholesterol is a structural component of biological membranes and its content affects membrane stability, permeability and protein mobility, and is required for the biosynthesis of sex hormones and bile acids. Triglycerides are a source of metabolic energy. The protein fraction of lipoproteins (also often called apolipoprotein, apolipoprotein) is mainly involved in lipid transport and metabolism and has important functions such as acute phase response, complement activation, immune response, inflammatory response, and protease inhibitor.
Creative Proteomics provides a platform for lipoprotein analysis that enables comprehensive analysis and determination of lipoprotein binding protein species and content, facilitating systematic study of lipoprotein structure and function, and analysis of the relationship between protein components in lipoproteins and lipid metabolism and related disease pathological processes.
Our Lipoproteins Analysis Service
- Accurate quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.
- Gel permeation HPLC: Separation of lipoproteins according to their hydrated particle size based on the principle of size exclusion chromatography (SEC).
- Isotope-dilution mass spectrometry (ID/MS): biomarkers, especially TG and TC measurements. Apolipoproteins are quantified by liquid chromatography ID/MS (LC-ID/MS).
- Liquid chromatography-tandem mass spectrometry: liquid chromatography coupled with triple quadrupole mass spectrometers for lipoproteins analysis service (Triple Quad™ 6500 LC-MS/MS System).
- MALDI-TOF-MS: Determination of the molecular mass of intact proteins in lipoproteins.
Fig 1. The workflow of lipoproteinss analysis service.
Our Detectable Lipoproteins Include But Are Not Limited To:
| Lipoprotein(a) [Lp(a)] | Low-density lipoprotein (LDL) | Oxidized lipoprotein(a) [oxLp(a)] | Oxidized low-density lipoprotein (ox-LDL) | Lipoprotein complexes (LDL-IC) |
| Large buoyant LDL (lbLDL) | Small dense LDL (sdLDL) | High-densitylipoproteincholesterol (HDL-C) | Very low density lipoprotein (VLDL) | Cholesteryl ester transfer protein (CETP) |
Sample Preparation
We accept a variety of samples, including but not limited to serum and plasma, etc. If you want to provide other samples, please contact our technicians.
- Serum/plasma and other body fluid samples: ≥ 100ul/sample
After quick freezing in liquid nitrogen, store at -80°C and send with dry ice.
Why Choose Us?
- One-stop analysis service: Provide comprehensive services from sample preparation, standard addition, mass spectrometry analysis, data processing, and data comparison.
- High sensitivity: High-sensitivity mass spectrometer is used to effectively detect low-level metabolites in the sample.
- Fast turnaround time: 2-6 weeks.
- Detailed report: Provide a detailed report at the end of the entire project, including the experimental process, MS/MS instrument parameters, quality control parameters, raw data and the final result of the comparison.
If you have any questions about our lipoproteins analysis services, please contact us.
* Our services can only be used for research purposes and Not for clinical use.
Resource:
Services:
Platform:
