Acylceramides Analysis Service

Creative Proteomics has developed a reliable and reproducible method using highly sensitive LC-MS/MS method for the rapid identification and quantification of acylceramides in skin sample, which can satisfy the needs of academic and industrial study in your lab.


A large number of ichthyosis subtypes have genetic causes and pathomechanisms associated with barrier malfunction in the stratum corneum, due to the aberrant synthesis, metabolism, and transport of functional lipids. The epidermis-specific lipid acylceramide plays a pivotal role in the formation of the permeability barrier in the skin. Abrogation of its synthesis causes the skin disorder ichthyosis. There are four classes of acylceramides in the epidermis: EOS, EOH, EOP, and EODS. Mainly, acylceramides form the skin barrier, and the EOS class of ceramide (CerEOS) with ultra-long-chain (ULC) fatty acid is the essential component of the stratum corneum barrier structures.

The structure of CerEOSFig1. The structure of CerEOS (Akiyama, Masashi, 2021)

Acylceramides samples, collected by tape stripping of human skin, were analyzed by reversed-phase liquid chromatography coupled to high-resolution quadrupole time-of-flight mass spectrometry operated in both positive and negative electrospray ionization mode in Creative Proteomics. Tape stripping is an almost non-invasive method with the benefits of controlling the sampling depth and collecting comparable sample amounts. This enables collecting a high number of samples and allows screening and biomarker studies, increasing much needed statistical validity of results and conclusions. All known classes of acylceramides could be measured in a repeatable manner.

Advantages of Our Acylceramides Analysis Service

  • One-stop analysis services, from sample preparation, mass spectrometry, data processing, to the identification and quantification of lipids.
  • Accurate quantification of skin acylceramides with high resolution UPLC-LC-MS.
  • Creative Proteomics full-service facility provides data processing (e.g., data alignment, feature detection), feature prioritization (e.g., principal component analysis, heat maps, ANOVA/t-test, multivariate statistical analysis, self organizing maps, volcano plots, and network activity prediction), and lipid identification services.

List of Detectable Acylceramides at Creative Proteomics

The Technical Process of Our Acylceramides Analysis Service

The workflow of acylceramides analysis serviceThe workflow of acylceramides analysis service

Structural details: species level
Precision: pmol & mol%
Variants identified: >250
Delivery time: 2-4 weeks

Applications of Acylceramides Analysis

  • Skin aging research
  • Cosmetic claim support
  • Topical drug development, efficacy testing for dermatological pharmacology and the cosmetic industry, as well as for academic researchers.


  • Experimental procedure
  • Parameters of liquid chromatography and MS
  • Purity analysis report
  • MS raw data files and MS data quality checks
  • Hexylceramide quantitative result data
  • Custom analysis report

Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, we provide reliable, fast and cost-effective acylceramides analysis services. If you have any questions about our acylceramides targeted lipidomics services, please contact us.


  1. Akiyama, Masashi. Acylceramide is a key player in skin barrier function: insight into the molecular mechanisms of skin barrier formation and ichthyosis pathogenesis. The FEBS Journal. 2021, 288.7: 2119-2130.
* Our services can only be used for research purposes and Not for clinical use.




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