Creative Proteomics has developed a reliable and reproducible method using highly sensitive LC-MS/MS method for the rapid identification and quantification of acylceramides in skin sample, which can satisfy the needs of academic and industrial study in your lab.
A large number of ichthyosis subtypes have genetic causes and pathomechanisms associated with barrier malfunction in the stratum corneum, due to the aberrant synthesis, metabolism, and transport of functional lipids. The epidermis-specific lipid acylceramide plays a pivotal role in the formation of the permeability barrier in the skin. Abrogation of its synthesis causes the skin disorder ichthyosis. There are four classes of acylceramides in the epidermis: EOS, EOH, EOP, and EODS. Mainly, acylceramides form the skin barrier, and the EOS class of ceramide (CerEOS) with ultra-long-chain (ULC) fatty acid is the essential component of the stratum corneum barrier structures.
Fig1. The structure of CerEOS (Akiyama, Masashi, 2021)
Acylceramides samples, collected by tape stripping of human skin, were analyzed by reversed-phase liquid chromatography coupled to high-resolution quadrupole time-of-flight mass spectrometry operated in both positive and negative electrospray ionization mode in Creative Proteomics. Tape stripping is an almost non-invasive method with the benefits of controlling the sampling depth and collecting comparable sample amounts. This enables collecting a high number of samples and allows screening and biomarker studies, increasing much needed statistical validity of results and conclusions. All known classes of acylceramides could be measured in a repeatable manner.
The workflow of acylceramides analysis service
Structural details: species level
Precision: pmol & mol%
Variants identified: >250
Delivery time: 2-4 weeks
Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, we provide reliable, fast and cost-effective acylceramides analysis services. If you have any questions about our acylceramides targeted lipidomics services, please contact us.