Serum and plasma are two distinct matrices separated from blood after phlebotomy. Serum is prepared from whole blood following a clotting process. Because of the differences in the preparation of the two blood matrices, lipid levels are expected to differ between plasma and serum, but both are excelente matrices. Serum untargeted lipidomics will not only provide insights into the specific functions of lipid species in health and disease, but will also identify potential biomarkers for establishing preventive or therapeutic programs for human diseases.
Identification the lipid profiles of serum can offer novel biomarkers for specific disease, also provide new insights for us to deeply understand the pathogenic mechanism of diseases. Creative Proteomics has established a technical platform for serum lipidomics research by using high-resolution mass spectrometer. According to your different experimental purposes, the corresponding sample pretreatment program is adopted, which significantly improves the accuracy of lipidomics analysis of serum samples.
Creative Proteomics is based on the liquid-mass spectrometry (LC-MS) unbiased detection of all lipid molecules in serum samples as much as possible. We use statistical analysis to screen differential lipid molecules, discover the relative relationship between lipid metabolism changes and physiological and pathological changes, and reveal the mechanism of lipids in various life activities.
Fig 1. The workflow of serum untargeted lipidomics.
According to different experimental schemes, we usually need 100-200 ul serum for our untargeted lipidomics services.
Since serum samples need to be coagulated, make sure that all samples have the same clotting time. Avoid repetitive freeze-thaw cycles, store at -80°C.
If you have any questions about our exosomes lipidomics service, please contact us.