Endocannabinoids Analysis Service

Creative Proteomics has developed a reliable and reproducible method using highly sensitive GC/LC-MS/MS method for the rapid identification and quantification of endocannabinoids in different sample types, which can satisfy the needs of academic and industrial study in your lab.

Overview

Endocannabinoids are a family of lipid messengers present in a wide range of living organisms. In the brain, they regulate ion‐channel activity and neurotransmitter release critical to biological processes such as synaptic plasticity and learning and memory. Endocannabinoids are embedded within an intricate network of lipid pathways, the regulation of which controls the strength and duration of their signaling. Therefore, physiological, pathological, or pharmacological perturbations of these interconnected lipid pathways have a profound effect on the regulation of endocannabinoid signaling.

Structures of AEA and 2-AG glycerol, the two best known endocannabinoidsFig 1. Structures of AEA and 2-AG glycerol, the two best known endocannabinoids (Keereetaweep, J.; Kent D. Chapman,2016)

The recent development of high‐sensitivity and high‐throughput analytical tools affords a broader view of the endocannabinoid system, allowing researchers to place individual endocannabinoid molecules in the context of the interconnected network of their precursors and derivatives. Targeted lipidomics provides new opportunities for understanding endocannabinoid metabolism.

Gas chromatography (GC) has been widely used to separate endocannabinoid metabolites in lipid extracts from biological samples. GC is most often coupled with MS for sensitive detection and quantification of the analytes. In liquid chromatography- (LC-) based analysis of endocannabinoid metabolites, the stationary phase is usually reverse phase.

Advantages of Our Endocannabinoids Analysis Service

  • Creative Proteomics provides comprehensive services from prepare the samples, run the mass spectrometric analysis, process the data, perform statistical analysis, and identify the compounds.
  • The samples that can be analyzed cover a wide range, including subcellular compartments, cultured cells, blood and plasma, tissues and organs: liver, brain etc. Still, our services require only minimal sample amounts per analysis.
  • A detailed report will be provided at the end of the whole project, including the experiment procedure, MS/MS instrument parameter, raw data and data reports with figures, ready to be published.

Our Endocannabinoids Analysis Service

The workflow of endocannabinoids targeted lipidomicsFig 2. The workflow of endocannabinoids targeted lipidomics

Structural details: species level
Precision: pmol & mol%
Delivery time: 2-4 weeks
Sample requirements
Blood/plasma: 500 ul/sample
Tissue: 200 mg/sample
Cells: 1x107/sample

  • A minimum of 6 replicate plates or wells per condition are required.
  • After the sample is collected, it will be stored at -80°C immediately and transported by dry ice.
  • Tubes should be clearly labeled with sample identifiers and include a hard copy of the list of samples to accompany the shipment. Please include all available sample information when shipping samples.

Deliverables

  • Experimental procedure
  • Parameters of liquid chromatography and MS
  • Purity analysis report
  • MS raw data files and MS data quality checks
  • Hexylceramide quantitative result data
  • Custom analysis report

Because of their low abundance and structural variability, the quantification of endogenous endocannabinoid is challenging. With years of experience in lipidomics analysis services, Creative Proteomics has a good reputation in supporting the detection and quantification of a variety of lipids. Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with GC/LC-MS/MS, we provide reliable, fast and cost-effective endocannabinoids analysis services. If you have any questions about our endocannabinoids targeted lipidomics services, please contact us.

Reference:

  1. Keereetaweep, Jantana.; Kent D. Chapman. Lipidomic analysis of endocannabinoid signaling: targeted metabolite identification and quantification. Neural plasticity. 2016.

* Our services can only be used for research purposes and Not for clinical use.

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