Sphingosine Base Analysis Service

Sphingoid bases, also called "long-chain bases" are the backbone of all sphingolipids.

The main sphingoid bases of mammalian tissues are sphingosine (2S,3R-d-erythro-2-amino-1,3-octadec-4E-ene-diol), sphinganine (2S,3R-d-erythro-2-amino-1,3-octadecane diol) and 4-hydroxy-sphinganine (2S,3S,4R-erythro-2-amino-1,3,4-octadecane triol). In yeast, the main sphingoid bases are dihydrosphingosine and phytosphingosine.

Sphingoid base synthesis is required for the internalization step of endocytosis and organization of the actin cytoskeleton in yeast. Sphingoid bases have also been indicated to play a role in regulation of cell surface expression of amino acid permeases.

Biosynthesis of long chain bases in sphingolipidsFig 1 . Biosynthesis of long chain bases in sphingolipids (Mashima R et al, 2019)

Moreover, sphingoid bases were shown to affect signaling pathways such as protein kinase C and phosphatidic acid. In addition, it has been suggested that increased Sphingoid bases increase with aging in yeast. Sphingoid bases were demonstrated to mediate the aging process by inhibiting mitochondrial fusion and causing fragmentation, resulting in decreases in mtDNA copy numbers, ATP levels, mitochondrial membrane potentials, and oxygen consumption.

Creative Proteomics uses liquid chromatography mass spectrometry (LC-MS) method to identify the samples, which can effectively and accurately detect the changes in the content of sphingoid bases.

Our Sphingoid Bases Analysis Service

Creative proteomics uses a liquid chromatography triple quadrupole mass spectrometry analysis system (QTRAP 4500 LC-MS/MS System) to achieve qualitative and quantitative analysis of sphingoid bases.

The workflow of sphingoid bases analysis service.Fig 2. The workflow of sphingoid bases analysis service.

Sample Preparation

We can accept a variety of sample types, including cells, conditioned medium, subcellular compartments, exsome, tissue, blood, plasma, skinfor our customer.

After sample collection, you can either perform sample pretreatment, or contact us for specific sample pretreatment service.

Why Choose Us?

  • One-stop analysis service: Provide comprehensive services from sample preparation, standard addition, mass spectrometry analysis, data processing, and data comparison.
  • High sensitivity: High-sensitivity mass spectrometer is used to effectively detect low-level metabolites in the sample.
  • Fast turnaround time: 1-4 weeks.
  • Detailed report: Provide a detailed report at the end of the entire project, including the experimental process, MS/MS instrument parameters, quality control parameters, raw data and the final result of the comparison.

If you have any questions about our sphingosine base detection service, please contact us.

Reference:

  1. Mashima R.; et al. Biosynthesis of long chain base in sphingolipids in animals, plants and fungi. Future Science OA. 2019, 6(1): FSO434.
* Our services can only be used for research purposes and Not for clinical use.

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