LysoPAF Analysis Service

With extensive experience and state-of-the-art technologies, Creative Proteomics use highly sensitive LC-MS/MS method for the rapid identification and quantification of lysoPAF in different sample types, which can satisfy the needs of academic and industrial study.

Overview

Ether-linked lysophosphatidylcholine (lysoPAF or LPC O-) is a glycerophosphocholine, a type of glycerophospholipid. Their structure consists of a glycerol backbone connected to fatty alcohols through ether bonds and phosphorylcholine molecules. Fatty acids can be variable length, hydroxylated, and contain double bonds. Little is known about the biological functions of ether-linked lysophosphatidylcholine. They are the precursors of ether-linked phosphatidylcholine and platelet activating factor (PAF), which are important for the inflammatory response, as well as for the structure and function of the central nervous system. Elevated levels of LPC O-lipid have been found in patients with Alzheimer's disease.

LysoPAF Analysis Service

The combination of MS instrument and liquid chromatography (LC) can effectively separate target lipid molecules from complex samples. High-quality and accurate lipid level analysis helps to study the changes in the content of lysoPAF under physiological and other pathological conditions, and accelerate the analysis of biomarkers and related disease mechanisms.

Advantages of Our LysoPAF Analysis Service

  • One-stop services, including lipid extraction, lipid detection and data analysis. Cutting-edge apparatuses, reliable data and reproducible process.
  • Creative Proteomics designs experimental programs according to customer needs and chooses different technical methods to improve detection efficiency and ensure the accuracy and repeatability of experimental results.
  • Our project is a very complex study that requires a high degree of sensitivity and accuracy. Creative Proteomics has molecular biologists, mass spectrometry specialists, bioinformatics experts, and lipidomics data scientists. We are well-prepared and positioned to foster collaboration in the fast-developing field of lipidomics.

List of Detectable LysoPAF at Creative Proteomics

LPC O- 15:0;0LPC O-LPC O- 20:0;0

The Technical Process of Our LysoPAF Analysis Service

The workflow of lysoPAF analysis serviceFig 2. The workflow of lysoPAF analysis service

Structural details: species level
Precision: pmol & mol%
Variants identified: >25
Delivery time: 2-4 weeks
Sample requirements
Blood/plasma: 500 ul/sample
Tissue: 200 mg/sample
Cells: 1x107/sample

  • A minimum of 3 replicate plates or wells per condition are required.
  • After the sample is collected, it will be stored at -80°C immediately and transported by dry ice.
  • Tubes should be clearly labeled with sample identifiers and include a hard copy of the list of samples to accompany the shipment. Please include all available sample information when shipping samples.

Deliverables

  • Experimental procedure
  • Parameters of liquid chromatography and MS
  • Purity analysis report
  • MS raw data files and MS data quality checks
  • Hexylceramide quantitative result data
  • Custom analysis report

With years of experience in lipidomics analysis services, Creative Proteomics has a good reputation in supporting the detection and quantification of a variety of lipids. Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, we provide reliable, fast and cost-effective lysoPAF analysis services. If you have any questions about our lysoPAF targeted lipidomics services, please contact us.

* Our services can only be used for research purposes and Not for clinical use.

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