Prostaglandins (PG) are a group of lipid compounds with different physiological activities that have hormone-like effects in animals. Prostaglandins are found in almost all tissues and organs of humans and other animals. They are produced by almost all nucleated cells. Prostaglandins act on the autocrine and paracrine lipid mediators of endothelium, platelets, uterus and mast cells.

PGE2 is the most abundant PG in the body. It has inflammation manifestations such as fever and pain, as well as hyperpermeability of blood vessels. PGF2α regulates the functions of female reproductive organs, such as the degradation of the corpus luteum and the induction of labor pain. PGI2 reduces vascular tone by expanding vascular smooth muscle and anticoagulant effect. PGD2 can induce sleep and has anti-inflammatory effects in a variety of inflammatory diseases. TXB2 is a vasoconstrictor that promotes platelet aggregation.

Creative Proteomics provides comprehensive prostaglandins targeted lipidomics services from lipid extraction, lipid detection and data analysis for biomedical research institutions, biotechnology and pharmaceutical companies.

Fig 1. Synthesis of prostaglandins (Peebles Jr R S, 2019)

Our Prostaglandin Analysis Service

Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.

High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for prostaglandin analysis service (Triple Quad™ 6500 LC-MS/MS System).

Fig 2. The workflow of prostaglandin targeted lipidomics. (Creative Proteomics)

Sample Requirements

We can receive many types of samples, including cerebral tissues, urine, human menstrual fluid, plasma.

After sample collection, you can either perform sample pretreatment, or contact us for specific sample pretreatment service.

Detectable Prostaglandin Include But Are Not Limited To

Why Choose Us?

• High throughput: Only one injection can run both quantitative MRM and qualitative QTRAP scans to maximize throughput.
• One-stop analysis service: Provide comprehensive services from sample preparation, standard addition, mass spectrometry analysis, data processing, and data comparison.
• Fast turnaround time: 1-4 weeks.
• Provide a detailed report at the end of the entire project, including the experimental process, MS/MS instrument parameters, quality control parameters, raw data and the final result of the comparison.

Applications of Prostaglandin Analysis

• Identification potential biomarkers for establishing preventive or therapeutic programs for human diseases
• Study metabolic pathway mechanisms

If you have any questions about our lipidomics service, please contact us.

References:

1. Peebles Jr R S. Prostaglandins in asthma and allergic diseases. Pharmacology & therapeutics. 2019, 193: 1-19.
2. Pawelzik S C.; et al. Urinary prostaglandin D2 and E2 metabolites associate with abdominal obesity, glucose metabolism, and triglycerides in obese subjects. Prostaglandins & other lipid mediators. 2019, 145: 106361.

Services:

• Glycerophospholipids Analysis Service
• Glycerolipids Analysis Service
• Sphingolipids Analysis Service
• Fatty Acyls Analysis Service
• Sterol Lipids Analysis Service
• Phospholipids Analysis Service
• Prenol Lipids Analysis Service
• Saccharolipids Analysis Service
• Polyketides Analysis Service
• Untargeted Lipidomics
• Targeted Lipidomics
• Lipidomics Bioinformation Analysis
• Lipidomics Service

Resource:

Platform:

• Mass Spectrometry Systems
• Chromatographic Systems
• Shot-Gun Lipidomics
• GC/LC-MS Lipidomics
• Sample Guidelines