Mammalian, referring to mammals, constitutes a diverse and vital group within the biological classification. With characteristics such as vertebrate status and maternal nurturing, mammals hold a unique place in the animal kingdom. Their ecological roles range from predators and herbivores to seed dispersers, playing a crucial part in maintaining ecological balance. Mammals are not only indispensable in ecosystems but also hold immense significance for human life, providing food, fiber, companionship, and serving as model organisms for research and drug testing.
The field of lipidomics, focusing on the study of lipids within biological organisms, delves deep into the understanding of the composition, structure, and function of lipids in mammalian species.
Creative Proteomics provides mammalian lipidomics to accelerate your project. Our mammalian lipidomics service find applications in various research areas and purposes, including:
Lipid Profiling: Our Lipid Profiling service provides an in-depth analysis of the lipidome within mammalian samples. This includes the identification of lipid classes, individual lipid species, and their relative quantities. We tailor our profiling to your specific research objectives, ensuring a detailed overview of the lipid composition.
Lipid Quantification: We utilize advanced mass spectrometry techniques to quantify specific lipids within your samples accurately.
Lipid Structure Analysis: Our experts can elucidate the structural details of lipids, including fatty acid chain lengths and degrees of unsaturation.
Lipidomic Data Interpretation: Creative Proteomics doesn't just provide data; we offer in-depth interpretation and analysis of lipidomic results.
Fig1. The workflow of mammalian lipidomics.
Liquid Chromatography-Mass Spectrometry (LC-MS)
Our Agilent 6550 Q-TOF instrument is a powerful LC-MS system that provides high-resolution mass spectrometry data. It is particularly well-suited for the analysis of a diverse range of lipids within mammalian samples. The high-resolution capabilities of this instrument enable the accurate identification and quantification of lipid species.
Gas Chromatography-Mass Spectrometry (GC-MS)
Thermo Fisher ISQ LT is our choice for Gas Chromatography-Mass Spectrometry. This instrument excels in the separation and analysis of lipid compounds, particularly those requiring derivatization prior to analysis. It is highly sensitive and can provide quantitative information about lipids present in mammalian samples.
Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS)
For Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry, we employ the AB SCIEX TOF/TOF 5800 instrument. This technology is ideal for the analysis of a broad spectrum of lipid classes without the need for extensive sample preparation. The TOF/TOF configuration of this instrument offers exceptional sensitivity and resolution.
|Fatty Acids||Palmitic acid, Oleic acid, Linoleic acid, Stearic acid, Myristic acid, Arachidonic acid, Caprylic acid, Capric acid, Lauric acid, Eicosapentaenoic acid (EPA)|
|Triglycerides||Triolein, Tristearin, Trilinolein, Tripalmitin, Tricaprylin, Trilaurin, Trilinolenin, Tridecanoate, Tridecanoin, Triarachidin|
|Phospholipids||Phosphatidylcholine, Phosphatidylethanolamine, Phosphatidylserine, Phosphatidylinositol, Phosphatidic acid, Cardiolipin, Lecithin, Sphingomyelin|
|Glycolipids||Gangliosides, Cerebrosides, Sulfolipids, Galactolipids, Glucosylceramides|
|Sterols||Cholesterol, Sitosterol, Ergosterol, Campesterol, Stigmasterol, Brassicasterol, Lanosterol|
|Sphingolipids||Sphingomyelin, Ceramide, Sphingosine, Cerebroside, Sulfatides, Globosides|
|Waxes||Beeswax, Carnauba wax, Jojoba wax, Candelilla wax, Lanolin, Montan wax|
|Lipid-Soluble Vitamins||Vitamin A (Retinol), Vitamin D (Cholecalciferol), Vitamin E (Tocopherol), Vitamin K (Phylloquinone), Vitamin F (Essential fatty acids)|
|Glycerides||Monoglycerides, Diglycerides, Monoolein, 1,2-Diolein, 1,3-Dipalmitin, 2,3-Distearin, Monoarachidin|
|Other Lipids||Lipoic acid, Betaine lipids, Terpenes, Steroids, Plasmalogens, Alkenylacylglycerol, Ether lipids, Lipopolysaccharides, Resolvins, Neuroprostanes|