Cardiolipins Analysis Service

Cardiolipin (CL) is a tetra-acylated dicardiolipin lipid found in bacteria, yeast, plants, and animals, which is exclusively located in mitochondria, and has a unique structure that consists of 2 phosphate residues and 4 kinds of fatty acyl chains.

Cardiolipin plays an important role in regulating various kinds of mitochondrial proteins such as electron transport complexes, carrier proteins and phosphate kinases, and is also essential for the organization of particular mitochondrial structures such as cristae and contact sites.

Dead signals, infection, or disease may change the level of CL saturation and oxidation and cause its translocation to the cytosolic side of the outer mitochondrial membrane (OMM), affecting mitochondrial function and the inflammatory response.

Synthesis and Structure of Cardiolipin

Creative proteomics provides reliable, rapid and cost-effective cardiolipin analysis based on highly stable, reproducible and sensitive separation, characterization, identification and quantification systems in combination with LC-MS/MS.

Our Cardiolipin Analysis Service

Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.

High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for cardiolipin analysis service (Triple Quad™ 6500 LC-MS/MS System).

The workflow of cardiolipin analysis serviceFig 2. The workflow of cardiolipin analysis service

Sample Requirements

We routinely cover a rich variety of sample types, including animal and plant tissues, microorganisms, serum, cells, cell medium, et al. for our customer.

The samples should be stored at -80°C and shipped on dry ice.

Why Choose Us?

  • Fast turnaround time: 1-4 weeks.
  • High throughput: Only one injection can run both quantitative MRM and qualitative QTRAP scans to maximize throughput.
  • One-stop analysis service: Provide comprehensive services from sample preparation, standard addition, mass spectrometry analysis, data processing, and data comparison.
  • Detailed report: Experimental process, MS/MS instrument parameters, quality control parameters, raw data and the final result of the comparison.
  • We will use our expertise to help you interpret the results and provide you with actionable recommendations on how to utilize these results in your research or business.

If you have any questions about our cardiolipin detection service, please contact us.

References:

  1. Pizzuto M.; Pelegrin P. Cardiolipin in Immune Signaling and Cell Death. Trends in Cell Biology, 2020.
  2. Liu, X.; G. Xu. Recent advances in using mass spectrometry for mitochondrial metabolomics and lipidomics - A review. Analytica Chimica Acta. 2018. 1037: p. 3-12.
* Our services can only be used for research purposes and Not for clinical use.

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