Cardiolipin (CL) is a tetra-acylated dicardiolipin lipid found in bacteria, yeast, plants, and animals, which is exclusively located in mitochondria, and has a unique structure that consists of 2 phosphate residues and 4 kinds of fatty acyl chains.
Cardiolipin plays an important role in regulating various kinds of mitochondrial proteins such as electron transport complexes, carrier proteins and phosphate kinases, and is also essential for the organization of particular mitochondrial structures such as cristae and contact sites.
Dead signals, infection, or disease may change the level of CL saturation and oxidation and cause its translocation to the cytosolic side of the outer mitochondrial membrane (OMM), affecting mitochondrial function and the inflammatory response.
Creative proteomics provides reliable, rapid and cost-effective cardiolipin analysis based on highly stable, reproducible and sensitive separation, characterization, identification and quantification systems in combination with LC-MS/MS.
Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.
High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for cardiolipin analysis service (Triple Quad™ 6500 LC-MS/MS System).
Fig 2. The workflow of cardiolipin analysis service
We routinely cover a rich variety of sample types, including animal and plant tissues, microorganisms, serum, cells, cell medium, et al. for our customer.
The samples should be stored at -80°C and shipped on dry ice.
If you have any questions about our cardiolipin detection service, please contact us.