The intricate process of fatty acid oxidation plays a fundamental role in the generation of energy by breaking down fatty acids into acetyl-CoA, which then enters the Krebs cycle. This pathway transpires within the mitochondria of cells and its regulation holds paramount significance in maintaining the requisite equilibrium of energy metabolism. Moreover, the metabolites engendered during this pathway have been demonstrated to perform pivotal functions in diverse physiological processes, encompassing inflammation, insulin sensitivity, and lipid homeostasis. In light of the pathway's significance, the analysis of fatty acid oxidation and its metabolites is an indispensable endeavor that facilitates comprehension of metabolic diseases, such as obesity, diabetes, and cardiovascular disease.
Fatty acid oxidation is a process by which fatty acids are broken down to produce energy. This process occurs in the mitochondria of the cell and is regulated by a series of enzymes, transporters and receptors. Fatty acids are first activated by acyl-CoA synthase, which attaches CoA to fatty acids to form acyl-CoA. acyl-CoA molecules are then transported to the mitochondria by the carnitine shuttle system. Once in the mitochondria, acyl-CoA is converted to acetyl-CoA through a series of reactions that are collectively referred to as β-oxidation. The acetyl-CoA molecule then enters the Krebs cycle, producing ATP, the energy currency of the cell.
During fatty acid oxidation, a number of metabolites are produced that are important for cellular signaling and homeostasis. These metabolites include acylcarnitine and acyl-CoA esters involved in mitochondrial function and lipid metabolism, the latter acting as signaling molecules that regulate gene expression and metabolic pathways.
Creative Proteomics offers you fatty acid oxidation and its metabolite analysis based on an LC-MS platform that provides accurate and precise metabolite quantification based on high-resolution mass spectrometry.
Our LC-MS platform includes the use of an Agilent 1290 Infinity LC system and a Q Exactive HF-X mass spectrometer. The liquid chromatography system enables high-resolution separation of metabolites, while the Q Exactive HF-X mass spectrometer provides high sensitivity and accuracy for metabolite quantification.
We recommend providing at least 50-100 mg of sample tissue or 50-100 µL of biofluid sample (plasma, serum, urine, etc.).
Please store the samples at -80°C until shipment to ensure sample integrity. Prior to shipment, please ensure that the samples are appropriately labeled and packaged to prevent any potential sample degradation during transportation.
If you have any questions about our fatty acid oxidation and its metabolites analysis services, please contact us.