Lysophosphatidic acid (LPA) is one of the active components of lysophospholipids (LP). LPA is found in a wide range of organisms from prokaryotes to eukaryotes and is an important extracellular signal transmitter and intracellular second messenger. lPA is a metabolic intermediate in the ab initio lipid synthesis pathway. Under normal physiological conditions, LPA has effects on cell proliferation, prevention of apoptosis, cell migration, cytokine and chemokine secretion, platelet aggregation, smooth muscle contraction, smooth muscle cell transformation, and axon retraction.
LPA species include saturated fatty acids (16:0, 18:0) and unsaturated fatty acids (16:1, 18:1, 18:2, 20:4) (5~10). And these LPA species exhibit different biological activities.
Fig 1. Biosynthetic pathways of LPA (Shizu Aikawa et al., 2015)
To better explore the regulatory function, role and related mechanisms of LPA, Creative Proteomics provides reliable, rapid and cost-effective lysophosphatidic acid analysis based on highly stable, reproducible and sensitive separation, characterization, identification and quantification systems in combination with LC-MS/MS.
Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.
High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for lysophosphatidic acid analysis service (Triple Quad™ 6500 LC-MS/MS System).
Fig 2. The workflow of lysophosphatidic acid analysis service
We can accept samples of animal and plant tissues, plasma, etc. The samples should be stored at -80°C and shipped on dry ice. If you provide other samples, please contact our technicians for specific quantities and treatment methods.
If you have any questions about our lysophosphatidic acid detection service, please contact us.