Lipids are essential metabolites that have many crucial cellular functions and can provide a direct readout of cellular metabolic status. The total lipid content in a cell is called a lipidome. Lipidomics is the study of lipidomes using the principles and techniques of analytical chemistry, and emerged in 2003 as an approach to study the metabolism of the cellular lipidome.The analytical power of, and new developments in, mass spectrometry (MS) have accelerated this emerging discipline.
Lipidomics enables us to study cellular metabolism by quantifying the changes of individual lipid classes, subclasses and molecular species that reflect metabolic differences. As the pathways and networks of lipid metabolism have been extensively studied, any changes in lipid amounts can simultaneously reveal variations in several enzymatic levels, activities and/or gene expression patterns.
Creative Proteomics has extensive experience in the field of lipidomics. We are dedicated to providing cutting-edge mass spectrometry (MS)-based lipidomics services for biomedical research institutions, biotechnology and pharmaceutical companies.
Untargeted Lipidomics is a discovery-based research approach focusing on detection and relative quantitation of known and unknown lipids in a given biological system. This technology is most advantageously used in comparative studies, to identify the changes in the lipid profile among two or more experimental sample groups. Creative Proteomics provides both shot-gun lipidomics and LC-MS for Untargeted Lipidomics research. As the field of lipidomics continues to mature, untargeted lipidomics is an important aspect of biomarker discovery in biological and clinical studies.
Learn MoreTargeted lipidomics involves the identification and quantification of defined groups of known lipids across large sample sets. Creative Proteomics offers a variety of targeted lipid analysis services in a compound class or pathway-specific manner for your lipidomics needs. We use liquid chromatography or gas chromatography hyphenated to triple quadrupole mass spectrometers for our targeted lipidomics services, to provide high-specificity, high-selectivity, and high-reproducibility detection and quantitation of hundreds of known lipids in diverse biological samples. As the field of lipidomics continues to mature, targeted lipidomics offers a promising route to disease diagnosis, therapeutic management and prognostic prediction in clinical applications.
Learn MoreTissues are the primary manifestation of a very broad spectrum of diseases and are therefore one of the main pillars in clinical research and diagnostics. The investigation of morphological and molecular tissue features is the basis of generating knowledge about clinicopathological entities, diagnostics, prediction, and prognosis. Lipid composition varies significantly from tissues to tissues and even differs within the various cells of an tissue. MALDI-imaging mass spectrometry (MALDI-IMS) can reveal the characteristic distribution of several kinds of lipids in various tissues. Creative Proteomics provides a well-established solution of MALDI-Imaging.
Learn MoreMetabolic flux analysis (MFA), an important analytical technique, is used to examine production and consumption rates of metabolites in a biological system. Creative Proteomics offers untargeted metabolic flux and targeted metabolic flux analysis services of lipids metabolism for our customers. We has developed a novel metabolic flux analysis platform, which allows for the determination of control structure and metabolic pathway regulation network.
Learn MoreA detailed report will be provided at the end of the whole project, including the experiment procedure, MS/MS instrument parameter, raw data and data reports with figures, ready to be published. Creative Proteomics uses Progenesis QI (Waters) and LipidSearch (Thermo) and MS DIAL for our Lipidomics Statistics.
Learn MoreInstrumentation
Waters ACQUITY UPLC H Class Plus System
Shimadzu Nexera X2 U-HPLC System
Thermo Scientific™ TRACE™ 1310 GC System
Waters Xevo G2-XS QTof Mass Spectrometry
Waters SYNAPT G2-Si HDMS
AB Sciex 6500 QTRAP
Thermo Orbitrap Elite™ Hybrid Ion Trap-Orbitrap Mass Spectrometer
Thermo Scientific TSQ 9000 Triple Quadrupole GC-MS/MS System
TriVersa Nanomate Nanoelectrospray Ion Source
Applications
Our lipidomics services can be applied to various fields, including but not limited to the biotech and pharma, disease research, food & nutrition, agriculture, and environmental toxicology.
- Pharma & Biotech: target discovery, identification of candidate drugs, biomarkers, etc
- Disease research: cancer, atherosclerosis, diabetes mellitus, alzheimer's disease, kidney disease, etc
- Food & Nutrition: food origin identification, food adulteration detection, food safety assurance, nutrition and health, etc
- Agriculture: plant abiotic stress research, plant-microbe interactions, plant growth and development, etc
- Environmental Toxicology: organic pollutants, heavy metals, radiation, etc
- Microbiology Research: Host-pathogen interactions, antibiotic resistance studies, drug development, fermentation condition optimization analysis
Our Advantages
- Our project is a very complex study that requires a high degree of sensitivity and accuracy. Honestly, we are surprised by technical expertise and professional platform in Creative Proteomics. They did provide us with the best data to meet our need.
- Creative Proteomics designs experimental programs according to customer needs and choose different technical methods to improve detection efficiency and ensure the accuracy and repeatability of experimental results.
- The samples that can be analyzed cover a wide range, including subcellular compartments, bacteria, yeast and other microorganisms to cultured cells, skin tapes, body fluids, blood and plasma, tissues and organs: liver, brain etc. as well as oils and milk, plants, foods, Chinese traditional medicine. Still, our services require only minimal sample amounts per analysis.
- Creative Proteomics full-service facility provides data processing (e.g., data alignment, feature detection), feature prioritization (e.g., principal component analysis, heat maps, ANOVA/t-test, multivariate statistical analysis, self organizing maps, volcano plots, and network activity prediction), and lipid identification services.
- A detailed report will be provided at the end of the whole project, including the experiment procedure, MS/MS instrument parameter, raw data and data reports with figures, ready to be published.
How we work in 7 easy steps
Creative Proteomics has extensive experience in the field of lipidomics. We are dedicated to providing cutting-edge mass spectrometry (MS)-based lipidomics services for biomedical research institutions, biotechnology and pharmaceutical companies. If you have any special needs for lipids analysis, please contact us. Let us know what you need and we will accommodate you. We look forward to working with you in the future.
* Our services can only be used for research purposes and Not for clinical use.
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