Plant oil is composed of long-chain hydrocarbons, and thus can also replace petroleum in many applications, such as feedstock for chemical and biofuel. So far, improvements in productivity and sowing larger areas have managed to keep pace with demand. However, finite agricultural land, increasing populations and more widespread use of crops for renewable chemicals/biofuels suggest that plant oils will soon be in short supply. Some studies have studied the regulation of oil accumulation in crops, partly by the application of flux control analysis. These experiments have revealed important overall characteristics of the process but often could not delineate some of the details. To elucidate some of these details and to further our knowledge of regulation, many studies have utilised lipidomics. Lipidomics as a novel technique can provide important data about lipid metabolism during oil accumulation.
Siberian apricot seed kernel (SASK) contains a high of 50% oil with suitable fuel properties conformed to biodiesel standard. The study conducted the detailed lipidomics examination of important lipid classes during SASK development. According to the temporal patterns of oil and FAs in developing SASK, the optimal samples from 4, 6, and 8 WAA that, respectively, represent early, middle, and late phases of oil accumulation were identified for lipidomics analysis.
The lipidomics results showed quite distinct molecular species distribution in PA, DAG, PC, PE, PG, and TAG, reflecting the complexity of lipid flux metabolism during oil accumulation. Moreover, the lipidomics data coupled with qRT‐PCR analysis indicated that PC is a central intermediate in the flux of PUFAs or DAG, or both substrates destined to TAG synthesis in developing SASK. All of these findings will be conducive to understand the underlying mechanisms how the storage lipid is formed in developing SASK.
Fig1. Changes to phospholipids during SASK development (Mai, Yiting.; et al, 2020)
These datas contribute significantly to understand the underlying mechanisms of lipid accumulation in plant seed, and may also present strategies for engineering oil accumulation in oilseed plants. With decades of operational experience and technology platform, Creative Proteomics provides reliable, rapid, and cost-effective plant lipidomics services based on LC-MS methods for lipid accumulation during seed development research.
If you have any questions about our lipidomics services in lipid accumulation during seed development research, please contact us. With our highly experienced scientific team, advanced techniques and equipment, we can tailor our services according to your needs.