Protectins D1 Analysis Service

Protectin D1 also known as neuroprotectin D1 (when it acts in the nervous system) and abbreviated most commonly as PD1 or NPD1. PD1 is an aliphatic acyclic alkene 22 carbons in length with two hydroxyl groups at the 10 and 17 carbon positions and one carboxylic acid group at the one carbon position.

Protectins D1 Targeted Lipidomics

PD1 is a member of the class of specialized proresolving mediators. Like other members of this class of polyunsaturated fatty acid metabolites, it possesses strong anti-inflammatory, anti-apoptotic and neuroprotective activity.

Creative Proteomics provides comprehensive protectins d1 targeted lipidomics services from lipid extraction, lipid detection and data analysis for biomedical research institutions, biotechnology and pharmaceutical companies.

Our Protectin D1 Analysis Service

Creative Proteomics used a triple quadrupole linear ion trap mass spectrometer equipped with a 1.7-μm, 1.0 × 150–mm Acquity UPLC BEH C18 column for protectins d1 targeted lipidomics.

The workflow of protectins d1 targeted lipidomicsFig 1. The workflow of protectins d1 targeted lipidomics

Sample Requirements

We routinely cover a rich variety of sample types, including cells, conditioned medium, exosome, serum, plasma for our customer.

Protectins d1 is unstable at room temperature, and the whole sample preparation procedure should be conducted in cold conditions whenever possible and, antioxidants could be used to minimize non-enzymatic oxidation.

Why Choose Us?

  • Fast turnaround time: 1-4 weeks.
  • High throughput: Only one injection can run both quantitative MRM and qualitative QTRAP scans to maximize throughput.
  • One-stop analysis service: Provide comprehensive services from sample preparation, standard addition, mass spectrometry analysis, data processing, and data comparison.
  • Data analysis including PCA, PLS-DA, PLS-R and univariate statistics, we generate a list of lipids that classifies data based on a selection of statistical tests: F-test, t-tests or regression and export lists for further analysis, such as enrichment and KEGG pathway analysis.
  • Detailed report: Experimental process, MS/MS instrument parameters, quality control parameters, raw data and the final result of the comparison.
  • We will use our expertise to help you interpret the results and provide you with actionable recommendations on how to utilize these results in your research or business.

If you have any questions about our protectins d1 targeted lipidomics services, please contact us.

Reference:

  1. Miyata J.; et al. Dysregulated synthesis of protectin D1 in eosinophils from patients with severe asthma[J]. Journal of allergy and clinical immunology. 2013, 131(2): 353-360. e2.
* Our services can only be used for research purposes and Not for clinical use.

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